State of differentiation of bovine epithelial lens cells in vitro. Relationship between the variation of the cell shape and the synthesis of crystallins

Correlations between cell morphology and the expression of specific proteins (crystallins) have been investigated. Two different culture conditions have been chosen which keep bovine epithelial lens cells (BEL cells) in a monolayer of homogeneous epithelioid cells: (1) bovine retinal extract (EDGF)...

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Veröffentlicht in:	Cell differentiation 1983-11, Vol.13 (3), p.185-190
Hauptverfasser:	Simonneau, Lionel, Herve, Bernadette, Jacquemin, Edith, Courtois, Yves
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cattle Cell Differentiation Cell morphology Cells, Cultured crystallin synthesis Crystallins - biosynthesis Culture Media Epithelial Cells Extracellular Matrix Fundamental and applied biological sciences. Psychology lens cells on extracellular matrix Lens, Crystalline - cytology Lens, Crystalline - metabolism Molecular and cellular biology
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Beschreibung
Zusammenfassung:	Correlations between cell morphology and the expression of specific proteins (crystallins) have been investigated. Two different culture conditions have been chosen which keep bovine epithelial lens cells (BEL cells) in a monolayer of homogeneous epithelioid cells: (1) bovine retinal extract (EDGF) supplemented medium; (2) extracellular matrix (ECM) provided by corneal endothelial cells in standard medium has been compared to previous results obtained with BEL cells cultivated on plastic (Simonneau et al., 1983). Variations of the cell shape had no effect upon crystallin synthesis.
ISSN:	0045-6039
DOI:	10.1016/0045-6039(83)90088-X