Effects of chlorcyclizine or phenobarbital on in vitro detoxication activity by larval wax moth gut homogenates
Chlorcyclizine dihydrochloride (0.5 g/100 g medium) or phenobarbital (1 g/100 g medium) incorporated into the media of 6th or 7th instar wax moth ( Galleria mellonella L.) larvae affected the rates of EPN detoxication, p-nitroanisole O-demethylation, and NADPH-neotetrazolium (NT)-reductase activity....
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Veröffentlicht in: | Toxicology and applied pharmacology 1971, Vol.18 (1), p.124-132 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Chlorcyclizine dihydrochloride (0.5 g/100 g medium) or phenobarbital (1 g/100 g medium) incorporated into the media of 6th or 7th instar wax moth (
Galleria mellonella L.) larvae affected the rates of EPN detoxication,
p-nitroanisole
O-demethylation, and NADPH-neotetrazolium (NT)-reductase activity. Neither drug significantly affected EPN detoxication or
O-demethylase with 2 days of treatment. However, after 3, 4, 5, 6, and 8 days of chlorcyclizine treatment, EPN detoxication activity by 6th instar gut homogenates increased to 238, 304, 234, 204, and 128% of the control, respectively. Similarly,
p-nitroanisole
O-demethylation was induced to 208, 504, 396, 265, and 290%. NADPH-NT-reductase was induced to 141, 156, 173, 182, 204, and 159% of the control with 2, 3, 4, 5, 6, and 8 days of chlorcyclizine treatment. Phenobarbital also induced these enzyme activities. The induction of EPN detoxication and
O-demethylase activity was much less in 7th than in 6th instar larvae, but NADPH-NT-reductase was more significantly induced in 7th than in 6th instar larvae. Both drugs, with up to 4 days of feeding, increased the gut protein, which then declined to less than the control values. The drug effects were dependent upon (1) days of drug treatment, (2) the drug used, (3) the enzyme studied, and (4) the instar of the insect. |
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ISSN: | 0041-008X 1096-0333 |
DOI: | 10.1016/0041-008X(71)90321-8 |