Preliminary crystallographic study of glucose-6-phosphate dehydrogenase from rat liver

Crystals of d-glucose-6-phosphate: NADP + oxidoreductase were obtained with the hanging drop, vapor diffusion and batch methods from ammonium sulfatecontaining solutions. X-ray diffraction photographs indicate that the crystals belong to the orthorhombic space groups I222 or I2 12 12 1 with unit cel...

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Veröffentlicht in:	Journal of molecular biology 1983-12, Vol.171 (2), p.233-236
Hauptverfasser:	Ammon, Herman L., Murphy, Kenan C., Bhattacharjee, Sovan K., Szepesi, Bela, Hansen, Robert J., Huber, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology glucose-6-phosphate dehydrogenase Glucosephosphate Dehydrogenase liver Liver - enzymology Male Molecular Weight Oxidoreductases Rats Rats, Inbred Strains X-Ray Diffraction
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Zusammenfassung:	Crystals of d-glucose-6-phosphate: NADP + oxidoreductase were obtained with the hanging drop, vapor diffusion and batch methods from ammonium sulfatecontaining solutions. X-ray diffraction photographs indicate that the crystals belong to the orthorhombic space groups I222 or I2 12 12 1 with unit cell dimensions of a=66·0 , b=140·8and c=177·8 . These data, together with results from sodium dodecyl sulfate/polyacrylamide gel electrophoresis and crystal density experiments, indicate that there is one 116,000 M r dimer per asymmetric unit. The crystals diffract to at least 2·2and are suitable for X-ray crystallographic structure determination.
ISSN:	0022-2836 1089-8638
DOI:	10.1016/S0022-2836(83)80357-X