Strain identification of Newcastle disease virus in tissue culture

A procedure was developed that enabled the investigator to isolate and to identify virus and virus type in one cultural step. The development and morphology of each virus was studied on monolayers of chicken embryo fibroblasts. The response of individual plaques were compared in several tests. Sampl...

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Veröffentlicht in:	Avian diseases 1970-11, Vol.14 (4), p.636-645
Hauptverfasser:	Beard, P.D, Spalatin, J, Hanson, R.P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Formation Chick Embryo Chickens Culture Techniques Elution Embryos Erythrocytes Fibroblasts Hemadsorption Hemadsorption Inhibition Tests Hemagglutination Tests Immune Sera Methods Neutralization Tests Newcastle disease virus Newcastle disease virus - analysis Newcastle disease virus - classification Newcastle disease virus - isolation & purification Newcastle disease virus - pathogenicity Rabbits Tissue culture techniques Vaccination Vaccines - analysis Viral morphology Viruses
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Beschreibung
Zusammenfassung:	A procedure was developed that enabled the investigator to isolate and to identify virus and virus type in one cultural step. The development and morphology of each virus was studied on monolayers of chicken embryo fibroblasts. The response of individual plaques were compared in several tests. Samples from seven of ten serials of vaccines contaminated with Newcastle disease virus (NDV) were found to contain a lentogenic strain of NDV. Six tissue-culture procedures were used. Six isolates were typed as NDV-LaSota-like, and one was typed as NDV-B1-like.
ISSN:	0005-2086 1938-4351
DOI:	10.2307/1588635