An enzyme-linked immunosorbent assay for antibody against acetylcholine receptor
An enzyme-linked immunosorbent assay is described for measurement of antibody against Torpedo acetylcholine receptor. As here developed, the assay is highly sensitive, reproducible, and requires small quantities of immunological reagents. Relative measurements of antibody concentration by this metho...
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| Veröffentlicht in: | Journal of neuroscience methods 1983-01, Vol.9 (2), p.141-155 |
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| container_end_page | 155 |
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| container_issue | 2 |
| container_start_page | 141 |
| container_title | Journal of neuroscience methods |
| container_volume | 9 |
| creator | Hinman, Channing L. Hudson, Richard A. Lynne Burek, C. Goodlow, Gerald Rauch, Helene C. |
| description | An enzyme-linked immunosorbent assay is described for measurement of antibody against
Torpedo acetylcholine receptor. As here developed, the assay is highly sensitive, reproducible, and requires small quantities of immunological reagents. Relative measurements of antibody concentration by this method are proportional to those determined by radioimmunoassay. |
| doi_str_mv | 10.1016/0165-0270(83)90127-9 |
| format | Article |
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Torpedo acetylcholine receptor. As here developed, the assay is highly sensitive, reproducible, and requires small quantities of immunological reagents. Relative measurements of antibody concentration by this method are proportional to those determined by radioimmunoassay.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/0165-0270(83)90127-9</identifier><identifier>PMID: 6358708</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>acetylcholine ; acetylcholine receptor ; Animals ; antibodies ; Autoantibodies - analysis ; Biological and medical sciences ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Immunoenzyme Techniques ; Kinetics ; myasthenia gravis ; Myasthenia Gravis - immunology ; Rabbits ; Radioimmunoassay ; Receptors, Cholinergic - immunology ; RIA ; Torpedo ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience methods, 1983-01, Vol.9 (2), p.141-155</ispartof><rights>1983</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-99887ea657c1f5a26b6706f7eb947d3fa0b9d2c1c7aab0c10af3e3903015ccae3</citedby><cites>FETCH-LOGICAL-c417t-99887ea657c1f5a26b6706f7eb947d3fa0b9d2c1c7aab0c10af3e3903015ccae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-0270(83)90127-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9388968$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6358708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hinman, Channing L.</creatorcontrib><creatorcontrib>Hudson, Richard A.</creatorcontrib><creatorcontrib>Lynne Burek, C.</creatorcontrib><creatorcontrib>Goodlow, Gerald</creatorcontrib><creatorcontrib>Rauch, Helene C.</creatorcontrib><title>An enzyme-linked immunosorbent assay for antibody against acetylcholine receptor</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>An enzyme-linked immunosorbent assay is described for measurement of antibody against
Torpedo acetylcholine receptor. As here developed, the assay is highly sensitive, reproducible, and requires small quantities of immunological reagents. Relative measurements of antibody concentration by this method are proportional to those determined by radioimmunoassay.</description><subject>acetylcholine</subject><subject>acetylcholine receptor</subject><subject>Animals</subject><subject>antibodies</subject><subject>Autoantibodies - analysis</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoenzyme Techniques</subject><subject>Kinetics</subject><subject>myasthenia gravis</subject><subject>Myasthenia Gravis - immunology</subject><subject>Rabbits</subject><subject>Radioimmunoassay</subject><subject>Receptors, Cholinergic - immunology</subject><subject>RIA</subject><subject>Torpedo</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r3DAQhkVoSLdp_0EKPpTSHJyOLFsfl0AIbRoIJIcWehNjeZyqtaWt5C24v77e7LLH5jDM4X3eYXgYO-NwwYHLj8s0JVQKPmhxboBXqjRHbMW1qkqp9PcXbHVAXrJXOf8EgNqAPGEnUjRagV6xh6tQUPg7j1QOPvyirvDjuAkxx9RSmArMGeeij6nAMPk2dnOBj-hDXiJH0zy4H3EpUpHI0XqK6TU77nHI9Ga_T9m3z5--Xn8p7-5vbq-v7kpXczWVxmitCGWjHO8brGQrFcheUWtq1YkeoTVd5bhTiC04DtgLEgYE8MY5JHHK3u_urlP8vaE82dFnR8OAgeImWw1KC17Vz4JcKKF4LRaw3oEuxZwT9Xad_Ihpthzs1rjd6rRbnVYL-2TcmqX2dn9_047UHUp7xUv-bp9jdjj0CYPz-YAZobWRW-xyh9Ei7Y-nZLPzFBx1flE72S76___xDxOnnZE</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Hinman, Channing L.</creator><creator>Hudson, Richard A.</creator><creator>Lynne Burek, C.</creator><creator>Goodlow, Gerald</creator><creator>Rauch, Helene C.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>An enzyme-linked immunosorbent assay for antibody against acetylcholine receptor</title><author>Hinman, Channing L. ; Hudson, Richard A. ; Lynne Burek, C. ; Goodlow, Gerald ; Rauch, Helene C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-99887ea657c1f5a26b6706f7eb947d3fa0b9d2c1c7aab0c10af3e3903015ccae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>acetylcholine</topic><topic>acetylcholine receptor</topic><topic>Animals</topic><topic>antibodies</topic><topic>Autoantibodies - analysis</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoenzyme Techniques</topic><topic>Kinetics</topic><topic>myasthenia gravis</topic><topic>Myasthenia Gravis - immunology</topic><topic>Rabbits</topic><topic>Radioimmunoassay</topic><topic>Receptors, Cholinergic - immunology</topic><topic>RIA</topic><topic>Torpedo</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hinman, Channing L.</creatorcontrib><creatorcontrib>Hudson, Richard A.</creatorcontrib><creatorcontrib>Lynne Burek, C.</creatorcontrib><creatorcontrib>Goodlow, Gerald</creatorcontrib><creatorcontrib>Rauch, Helene C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hinman, Channing L.</au><au>Hudson, Richard A.</au><au>Lynne Burek, C.</au><au>Goodlow, Gerald</au><au>Rauch, Helene C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An enzyme-linked immunosorbent assay for antibody against acetylcholine receptor</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>9</volume><issue>2</issue><spage>141</spage><epage>155</epage><pages>141-155</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>An enzyme-linked immunosorbent assay is described for measurement of antibody against
Torpedo acetylcholine receptor. As here developed, the assay is highly sensitive, reproducible, and requires small quantities of immunological reagents. Relative measurements of antibody concentration by this method are proportional to those determined by radioimmunoassay.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>6358708</pmid><doi>10.1016/0165-0270(83)90127-9</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of neuroscience methods, 1983-01, Vol.9 (2), p.141-155 |
| issn | 0165-0270 1872-678X |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | acetylcholine acetylcholine receptor Animals antibodies Autoantibodies - analysis Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors ELISA Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Immunoenzyme Techniques Kinetics myasthenia gravis Myasthenia Gravis - immunology Rabbits Radioimmunoassay Receptors, Cholinergic - immunology RIA Torpedo Vertebrates: nervous system and sense organs |
| title | An enzyme-linked immunosorbent assay for antibody against acetylcholine receptor |
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