An enzyme-linked immunosorbent assay for antibody against acetylcholine receptor

An enzyme-linked immunosorbent assay for antibody against acetylcholine receptor

An enzyme-linked immunosorbent assay is described for measurement of antibody against Torpedo acetylcholine receptor. As here developed, the assay is highly sensitive, reproducible, and requires small quantities of immunological reagents. Relative measurements of antibody concentration by this metho...

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Veröffentlicht in:Journal of neuroscience methods 1983-01, Vol.9 (2), p.141-155
Hauptverfasser: Hinman, Channing L., Hudson, Richard A., Lynne Burek, C., Goodlow, Gerald, Rauch, Helene C.
Format: Artikel
Sprache:eng
Schlagworte:
acetylcholine
acetylcholine receptor
Animals
antibodies
Autoantibodies - analysis
Biological and medical sciences
Central nervous system
Central neurotransmission. Neuromudulation. Pathways and receptors
ELISA
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Immunoenzyme Techniques
Kinetics
myasthenia gravis
Myasthenia Gravis - immunology
Rabbits
Radioimmunoassay
Receptors, Cholinergic - immunology
RIA
Torpedo
Vertebrates: nervous system and sense organs
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Beschreibung
Zusammenfassung:An enzyme-linked immunosorbent assay is described for measurement of antibody against Torpedo acetylcholine receptor. As here developed, the assay is highly sensitive, reproducible, and requires small quantities of immunological reagents. Relative measurements of antibody concentration by this method are proportional to those determined by radioimmunoassay.
ISSN:0165-0270
1872-678X
DOI:10.1016/0165-0270(83)90127-9