Distribution of restriction enzyme sites in the bovine parvovirus genome and comparison to other autonomous parvoviruses

Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061 and 1 University of Tennessee — Oak Ridge Graduate School of Biomedical Sciences and Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37803, U.S.A. A physical map has been constructed for bovine parvovirus (BPV) replicative form (RF) DNA synthesized in vitro . Analysis of restriction enzyme digestion products for 12 enzymes by neutral gel electrophoresis allowed the mapping of 26 cleavage sites between the 3' and 5' termini of the in vitro RF DNA. Cleavage sites for these enzymes were not detected within the 3'-terminal hairpin. Hin dIII, Sal I, Sma I, Sst II and Xor II did not cleave BPV DNA, while 12 other enzymes cleaved the genome at one to four sites. Eleven additional enzymes were shown to cleave the genome at 8 to 20 sites. Comparison of the restriction site locations for BPV with those of other autonomous parvoviruses suggests that there may be conservation within the genome of certain restriction sites. Keywords: bovine parvovirus, restriction sites, physical map Received 6 April 1983; accepted 12 July 1983.