T lymphoma variants with specifically altered growth in semi‐solid media

The relationship between the tumorigenic potential and the cloning efficiency of T lymphoma BW5147 in semi‐solid media has been studied. Two stable variants exhibiting a 30‐fold decrease of their cloning efficiencies in agarose an methycellulose media were independently isolated by negative selectio...

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Veröffentlicht in:	International journal of cancer 1983-11, Vol.32 (5), p.641-643
Hauptverfasser:	Claverie, Jean‐Michel, Cohn, Melvin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Division Cell Line Cell Separation Culture Media Hematologic and hematopoietic diseases Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphoma - pathology Medical sciences Methylcellulose Mice Neoplasm Transplantation Phenotype Sepharose
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Zusammenfassung:	The relationship between the tumorigenic potential and the cloning efficiency of T lymphoma BW5147 in semi‐solid media has been studied. Two stable variants exhibiting a 30‐fold decrease of their cloning efficiencies in agarose an methycellulose media were independently isolated by negative selection with FuDr. These variants show no alteration of their growth properties in liquid medium and are still able to proliferate in liquid suspension over a bottom layer of agarose. This new phenotype is not correlated with any decreased tumorigenicity in syngeneic AKR/J mice.
ISSN:	0020-7136 1097-0215
DOI:	10.1002/ijc.2910320520