pH dependency of the cyanide-nitroprusside colour reaction of cystine

pH dependency of the cyanide-nitroprusside colour reaction of cystine

Both the cyanide-nitroprusside colour reaction for cystine and cysteine and the Rothera test for acetone show a marked dependency on the degree of alkalinity of the final reaction mixture. It is recommended that urine specimens for screening by the cyanide-nitroprusside test should first be adjusted...

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Veröffentlicht in:Clinica chimica acta 1970-12, Vol.30 (3), p.737-740
Hauptverfasser: Georges, R.J., Politzer, W.M.
Format: Artikel
Sprache:eng
Schlagworte:
Acetone - analysis
Cyanides
Cysteine - analysis
Cysteine - urine
Cystine - analysis
Cystinuria - diagnosis
Ferricyanides
Humans
Hydrogen-Ion Concentration
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Zusammenfassung:Both the cyanide-nitroprusside colour reaction for cystine and cysteine and the Rothera test for acetone show a marked dependency on the degree of alkalinity of the final reaction mixture. It is recommended that urine specimens for screening by the cyanide-nitroprusside test should first be adjusted to pH 7–8 to ensure satisfactory conditions for colour development.
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(70)90268-8