Survival of synchronized Chinese hamster cells following freezing in liquid nitrogen

Survival of synchronized Chinese hamster cells following freezing in liquid nitrogen

Synchronized Chinese hamster cells were frozen in tissue culture medium with 15% glycerol added to −196 °C at various points in the cell cycle. The highest survival was obtained with cells frozen in M and late S, while the lowest survival was obtained with cells in G2. These differed by a factor of...

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Veröffentlicht in:Experimental cell research 1970-12, Vol.63 (2), p.476-477
Hauptverfasser: Koch, G.J., Kruuv, J., Bruckschwaiger, C.W.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cell Division
Cricetinae
Culture Techniques
Freezing
Methods
Nitrogen
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Zusammenfassung:Synchronized Chinese hamster cells were frozen in tissue culture medium with 15% glycerol added to −196 °C at various points in the cell cycle. The highest survival was obtained with cells frozen in M and late S, while the lowest survival was obtained with cells in G2. These differed by a factor of 2.2.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(70)90245-4