Inhibition of the mouse sperm surface alpha-D-mannosidase inhibits sperm-egg binding in vitro
In previous reports from this laboratory, we identified the presence of a novel alpha-D-mannosidase on the surface of rat, mouse, hamster, and human spermatozoa [J Cell Biol 1989; 109:1257-1267 and Biol Reprod 1990; 42:843-858]. Since it has been suggested that mannosyl residues on the egg zona pell...
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| Veröffentlicht in: | Biology of reproduction 1991-05, Vol.44 (5), p.913-921 |
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| creator | CORNWALL, G. A TULSIANI, D. R. P ORGEBIN-CRIST, M.-C |
| description | In previous reports from this laboratory, we identified the presence of a novel alpha-D-mannosidase on the surface of rat,
mouse, hamster, and human spermatozoa [J Cell Biol 1989; 109:1257-1267 and Biol Reprod 1990; 42:843-858]. Since it has been
suggested that mannosyl residues on the egg zona pellucida may be important for sperm-egg binding, studies were undertaken
to examine the potential role of the sperm alpha-D-mannosidase during fertilization. Incubation of mouse spermatozoa in the
presence of increasing concentrations of the inhibitory sugars, alpha-methyl mannoside, alpha-methyl glucoside, D-mannose,
or D-mannitol, resulted in a dose-dependent decrease in the number of spermatozoa bound per egg without a deleterious effect
on sperm motility or on the sperm acrosome, and a dose-dependent inhibition of the sperm mannosidase activity. Galactose,
however had no effect on sperm-egg binding or on sperm mannosidase activity. Two nucleotide sugars (UDP-GlcNAc and UDP-gal)
were also tested and shown to reduce sperm-egg binding but with only a minimal effect on sperm mannosidase activity. In additional
studies, spermatozoa incubated in the presence of a mannose-containing oligosaccharide exhibited a dramatic reduction in sperm-egg
binding that correlated with a similar inhibition of sperm mannosidase activity. The oligosaccharide substrate did not affect
sperm motility or the sperm acrosome. These studies suggest that the sperm alpha-D-mannosidase may play an important role
during fertilization. |
| doi_str_mv | 10.1095/biolreprod44.5.913 |
| format | Article |
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mouse, hamster, and human spermatozoa [J Cell Biol 1989; 109:1257-1267 and Biol Reprod 1990; 42:843-858]. Since it has been
suggested that mannosyl residues on the egg zona pellucida may be important for sperm-egg binding, studies were undertaken
to examine the potential role of the sperm alpha-D-mannosidase during fertilization. Incubation of mouse spermatozoa in the
presence of increasing concentrations of the inhibitory sugars, alpha-methyl mannoside, alpha-methyl glucoside, D-mannose,
or D-mannitol, resulted in a dose-dependent decrease in the number of spermatozoa bound per egg without a deleterious effect
on sperm motility or on the sperm acrosome, and a dose-dependent inhibition of the sperm mannosidase activity. Galactose,
however had no effect on sperm-egg binding or on sperm mannosidase activity. Two nucleotide sugars (UDP-GlcNAc and UDP-gal)
were also tested and shown to reduce sperm-egg binding but with only a minimal effect on sperm mannosidase activity. In additional
studies, spermatozoa incubated in the presence of a mannose-containing oligosaccharide exhibited a dramatic reduction in sperm-egg
binding that correlated with a similar inhibition of sperm mannosidase activity. The oligosaccharide substrate did not affect
sperm motility or the sperm acrosome. These studies suggest that the sperm alpha-D-mannosidase may play an important role
during fertilization.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod44.5.913</identifier><identifier>PMID: 1868148</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>alpha -mannosidase ; Animals ; Biological and medical sciences ; Carbohydrate Sequence ; Cell Membrane - enzymology ; eggs ; Female ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Male ; Mammalian male genital system ; Mannosidases - antagonists & inhibitors ; Mannosidases - physiology ; Mice ; Mice, Inbred Strains ; Molecular Sequence Data ; Morphology. Physiology ; Oligosaccharides - chemistry ; Sperm-Ovum Interactions - physiology ; Spermatozoa - enzymology ; Spermatozoa - physiology ; Substrate Specificity ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1991-05, Vol.44 (5), p.913-921</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5600554$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1868148$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CORNWALL, G. A</creatorcontrib><creatorcontrib>TULSIANI, D. R. P</creatorcontrib><creatorcontrib>ORGEBIN-CRIST, M.-C</creatorcontrib><title>Inhibition of the mouse sperm surface alpha-D-mannosidase inhibits sperm-egg binding in vitro</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>In previous reports from this laboratory, we identified the presence of a novel alpha-D-mannosidase on the surface of rat,
mouse, hamster, and human spermatozoa [J Cell Biol 1989; 109:1257-1267 and Biol Reprod 1990; 42:843-858]. Since it has been
suggested that mannosyl residues on the egg zona pellucida may be important for sperm-egg binding, studies were undertaken
to examine the potential role of the sperm alpha-D-mannosidase during fertilization. Incubation of mouse spermatozoa in the
presence of increasing concentrations of the inhibitory sugars, alpha-methyl mannoside, alpha-methyl glucoside, D-mannose,
or D-mannitol, resulted in a dose-dependent decrease in the number of spermatozoa bound per egg without a deleterious effect
on sperm motility or on the sperm acrosome, and a dose-dependent inhibition of the sperm mannosidase activity. Galactose,
however had no effect on sperm-egg binding or on sperm mannosidase activity. Two nucleotide sugars (UDP-GlcNAc and UDP-gal)
were also tested and shown to reduce sperm-egg binding but with only a minimal effect on sperm mannosidase activity. In additional
studies, spermatozoa incubated in the presence of a mannose-containing oligosaccharide exhibited a dramatic reduction in sperm-egg
binding that correlated with a similar inhibition of sperm mannosidase activity. The oligosaccharide substrate did not affect
sperm motility or the sperm acrosome. These studies suggest that the sperm alpha-D-mannosidase may play an important role
during fertilization.</description><subject>alpha -mannosidase</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Sequence</subject><subject>Cell Membrane - enzymology</subject><subject>eggs</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mannosidases - antagonists & inhibitors</subject><subject>Mannosidases - physiology</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Molecular Sequence Data</subject><subject>Morphology. Physiology</subject><subject>Oligosaccharides - chemistry</subject><subject>Sperm-Ovum Interactions - physiology</subject><subject>Spermatozoa - enzymology</subject><subject>Spermatozoa - physiology</subject><subject>Substrate Specificity</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UtLxDAQB_AgyrqufgFB6EG9dU2aR5ujrK-FBS96lJIm020kfZh0Xfz2Brp49TSH_28GZgahS4KXBEt-V9neeRh8bxhb8qUk9AjNCc9kmmeiOEZzjLFIKRX0FJ2F8IkxYTSjMzQjhSgIK-boY901trKj7bukr5OxgaTtdwGSMIBvk7DztdKQKDc0Kn1IW9V1fbBGRWGnzjDRFLbbpLKdsd02Rsm3HX1_jk5q5QJcHOoCvT89vq1e0s3r83p1v0mbjJAxBWI4IbzCRmUk1yCMZkwLk2FiaM7qqi7ywgCutcwIxRXlQnDNBMNCaoCKLtDtNDfe4msHYSxbGzQ4pzqI25QFzinlMvsXEi65pIWM8OoAd1ULphy8bZX_KQ-Hi_n1IVdBK1d71Wkb_hgXGHPOIruZWGO3zd56KEOrnItDabnf7xkreRnfRn8BTIKMJQ</recordid><startdate>199105</startdate><enddate>199105</enddate><creator>CORNWALL, G. A</creator><creator>TULSIANI, D. R. P</creator><creator>ORGEBIN-CRIST, M.-C</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199105</creationdate><title>Inhibition of the mouse sperm surface alpha-D-mannosidase inhibits sperm-egg binding in vitro</title><author>CORNWALL, G. A ; TULSIANI, D. R. P ; ORGEBIN-CRIST, M.-C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h211t-e1d5115b0da217ce6dc44c6d201d374fbf878de0fc92130b35665c464069ceeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>alpha -mannosidase</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Sequence</topic><topic>Cell Membrane - enzymology</topic><topic>eggs</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mannosidases - antagonists & inhibitors</topic><topic>Mannosidases - physiology</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Molecular Sequence Data</topic><topic>Morphology. Physiology</topic><topic>Oligosaccharides - chemistry</topic><topic>Sperm-Ovum Interactions - physiology</topic><topic>Spermatozoa - enzymology</topic><topic>Spermatozoa - physiology</topic><topic>Substrate Specificity</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CORNWALL, G. A</creatorcontrib><creatorcontrib>TULSIANI, D. R. P</creatorcontrib><creatorcontrib>ORGEBIN-CRIST, M.-C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CORNWALL, G. A</au><au>TULSIANI, D. R. P</au><au>ORGEBIN-CRIST, M.-C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of the mouse sperm surface alpha-D-mannosidase inhibits sperm-egg binding in vitro</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1991-05</date><risdate>1991</risdate><volume>44</volume><issue>5</issue><spage>913</spage><epage>921</epage><pages>913-921</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>In previous reports from this laboratory, we identified the presence of a novel alpha-D-mannosidase on the surface of rat,
mouse, hamster, and human spermatozoa [J Cell Biol 1989; 109:1257-1267 and Biol Reprod 1990; 42:843-858]. Since it has been
suggested that mannosyl residues on the egg zona pellucida may be important for sperm-egg binding, studies were undertaken
to examine the potential role of the sperm alpha-D-mannosidase during fertilization. Incubation of mouse spermatozoa in the
presence of increasing concentrations of the inhibitory sugars, alpha-methyl mannoside, alpha-methyl glucoside, D-mannose,
or D-mannitol, resulted in a dose-dependent decrease in the number of spermatozoa bound per egg without a deleterious effect
on sperm motility or on the sperm acrosome, and a dose-dependent inhibition of the sperm mannosidase activity. Galactose,
however had no effect on sperm-egg binding or on sperm mannosidase activity. Two nucleotide sugars (UDP-GlcNAc and UDP-gal)
were also tested and shown to reduce sperm-egg binding but with only a minimal effect on sperm mannosidase activity. In additional
studies, spermatozoa incubated in the presence of a mannose-containing oligosaccharide exhibited a dramatic reduction in sperm-egg
binding that correlated with a similar inhibition of sperm mannosidase activity. The oligosaccharide substrate did not affect
sperm motility or the sperm acrosome. These studies suggest that the sperm alpha-D-mannosidase may play an important role
during fertilization.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>1868148</pmid><doi>10.1095/biolreprod44.5.913</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1991-05, Vol.44 (5), p.913-921 |
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| subjects | alpha -mannosidase Animals Biological and medical sciences Carbohydrate Sequence Cell Membrane - enzymology eggs Female Fundamental and applied biological sciences. Psychology In Vitro Techniques Male Mammalian male genital system Mannosidases - antagonists & inhibitors Mannosidases - physiology Mice Mice, Inbred Strains Molecular Sequence Data Morphology. Physiology Oligosaccharides - chemistry Sperm-Ovum Interactions - physiology Spermatozoa - enzymology Spermatozoa - physiology Substrate Specificity Vertebrates: reproduction |
| title | Inhibition of the mouse sperm surface alpha-D-mannosidase inhibits sperm-egg binding in vitro |
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