The sedimentation of mixed cultures used in the treatment of effluents generated from terrestrial fuel distribution terminals

This study evaluated the use of coagulants (ferric chloride and aluminium sulphate) and an anionic polyelectrolyte (polyacrylamide) in the settling of a mixed culture (C1), which was used for the biodegradation of hydrocarbons present in effluent of fuel distribution terminals contaminated with diesel oil and gasoline. In preliminary investigations conducted in jar tests, the optimal concentrations of coagulant were obtained for further studies. After preliminary tests, biodegradation was evaluated in a central composite design (CCD) with varying concentrations of ferric chloride and polyelectrolyte. Ferric chloride and polyelectrolyte concentrations ranged from 77.9 to 422.12mg/L and 0.0 to 3.2mg/L, respectively. The responses monitored in CCD experiments included the sludge volume index (SVI), turbidity, and specific rate of oxygen uptake (SOUR), where values of 100mL/g, 840 nephelometric turbidity unit (NTU) and 58mgO2/gh, respectively, were obtained. Subsequently, biodegradation was monitored in a sequencing batch reactor (SBR). The results indicated that within five cycles, total petroleum hydrocarbon (TPH) removal increased from 75±1.0% to 79±0.5%, while the volatile suspended solids (VSS) increased from 1300 to 2500mg/L.