Phorbol esters induce translocation of the nPKC p105 to membrane in mussel hemocytes

Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced...

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Veröffentlicht in:	Molecular and cellular biochemistry 2003-08, Vol.250 (1-2), p.41-45
Hauptverfasser:	Mercado, Luís, Cao, Asunción, Barcia, Ramiro, Ramos-Martinez, Juan Ignacio
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bivalvia - metabolism Blotting, Western Cell Membrane - metabolism Cytosol - metabolism Down-Regulation Enzymes Esters Fluorescent Antibody Technique, Indirect Hemocytes - enzymology Hemocytes - metabolism Immunity Marine Microscopy, Confocal Mollusca Mollusks Phorbol Esters - pharmacology Protein Kinase C - chemistry Protein Kinase C - metabolism Protein Transport - drug effects Rodents Time Factors Translocation
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Zusammenfassung:	Previous works revealed the presence of the nPKC enzyme p105 in hemocytes of M. galloprovincialis Lmk. Specific mussel antibodies were obtained from mouse and used in confocal microscopy and Western blotting. These techniques allowed the observation of p105 cytosol-to-membrane translocation induced by TPA for the first time in hemocytes of molluscs. The incubation of mussel immune cells with TPA for longer than 30 min also triggered a down-regulation process. Mussel hemocytes are an excellent model to study the molecular processes of innate immunity.
ISSN:	0300-8177 1573-4919
DOI:	10.1023/A:1024981814050