Mapping of sequence-specific markers and loci controlling preharvest sprouting and alpha-amylase activity in rye (Secale cereale L.) on the genetic map of an F sub(2) (S120xS76) population

Location of the loci that control preharvest sprouting and alpha-amylase activity in rye was studied based on intercross S120xS76, consisting of 110 genotypes of F sub(2) and F sub(3) progenies. The genetic map currently consists of 141 loci distributed in 11 linkage groups, covering a distance of 506.4 cM, and was enriched during this study with 24 sequence-specific markers (7 SCARs, 7 SSRs, and 10 STSs). The extended map was applied for composite interval mapping of the loci controlling preharvest sprouting and a-amylase activity, revealing 3 significant QTLs for preharvest sprouting, located on chromosomes 3R, 5R and 6R (in 1999), and one QTL for a-amylase activity found on chromosome 2R (in 2000).