Quantitation of virus using laser-based scanning of near-infrared fluorophores replaces manual plate reading in a virus titration assay

A method was developed for quantitation of a virus titration assay for minimally cytopathic and noncytopathic viruses that utilizes laser-based scanning of near-infrared (NIR) fluorophores. This automated method bypasses the need for manual plate reading thus eliminating human bias and error. The im...

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Veröffentlicht in:	Journal of virological methods 2010-09, Vol.168 (1), p.57-62
Hauptverfasser:	Weldon, Sally K., Mischnick, Shawn L., Urlacher, Teresa M., Ambroz, Kristi L.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Automation - methods Biological and medical sciences BVDV Cell Line Computer programs Feline Panleukopenia Virus - isolation & purification FPV Fundamental and applied biological sciences. Psychology Infectivity assay Lasers Microbiology Near-infrared fluorescence Odyssey Techniques used in virology Viral Load - methods Virology
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Zusammenfassung:	A method was developed for quantitation of a virus titration assay for minimally cytopathic and noncytopathic viruses that utilizes laser-based scanning of near-infrared (NIR) fluorophores. This automated method bypasses the need for manual plate reading thus eliminating human bias and error. The image data is translated by LI-COR's Odyssey software into numerical data which is used directly in the virus titer calculations.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/j.jviromet.2010.04.016