INDUCTION OF LIVER MICROSOMAL 2-HYDROXYLATION OF ESTRADIOL 17-SULFATE BY PHENOBARBITAL IN MALE RAT

Estradiol 17-sulfate 2-hydroxylase activity was measured in liver microsomes of 7-to 8-weeks-old Wistar male rats. Phenobarbital (80 mg/kg, daily for 4 d) injected intraperitoneally caused 30% increase of the enzyme activity and 160% increase of the microsomal cytochrome P-450 content. Treatment of...

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Veröffentlicht in:	Journal of Pharmacobio-Dynamics 1983, Vol.6(6), pp.438-440
Hauptverfasser:	WATANABE, KAZUHIRO, YOSHIZAWA, ITSUO
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Chromatography, High Pressure Liquid - methods Cobalt - pharmacology Cytochrome P-450 Enzyme System - metabolism Estradiol - metabolism Hormones. Endocrine system Hydroxylation Male Medical sciences Methylcholanthrene - pharmacology Microsomes, Liver - metabolism Pharmacology. Drug treatments Phenobarbital - pharmacology phenobarbital-induced hydroxylation Rats Rats, Inbred Strains
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Zusammenfassung:	Estradiol 17-sulfate 2-hydroxylase activity was measured in liver microsomes of 7-to 8-weeks-old Wistar male rats. Phenobarbital (80 mg/kg, daily for 4 d) injected intraperitoneally caused 30% increase of the enzyme activity and 160% increase of the microsomal cytochrome P-450 content. Treatment of rats with 3-methylcholanthrene (40 mg/kg, daily for 4 d) injected intraperitoneally caused no induction effect on 2-hydroxylase activity, but P-450 content was increased by 113%. Both the P-450 content and the enzyme activity were reduced to about half of the control, when the animals were injected subcutaneously with cobaltous chloride (30 mg/kg, daily for 2 d).
ISSN:	0386-846X 1881-1353
DOI:	10.1248/bpb1978.6.438