Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida
A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60 771 Da.
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| Veröffentlicht in: | FEBS letters 1991-07, Vol.286 (1-2), p.55-57 |
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| container_end_page | 57 |
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| container_issue | 1-2 |
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| container_title | FEBS letters |
| container_volume | 286 |
| creator | Fessenmaier, Martin Frank, Rainer Retey, János Schubert, Carsten |
| description | A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60 771 Da. |
| doi_str_mv | 10.1016/0014-5793(91)80938-Y |
| format | Article |
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Psychology ; Genes, Bacterial ; Histidine - metabolism ; hutU Gene ; Lyases ; Molecular Sequence Data ; Multigene Family ; PCR ; Polymerase Chain Reaction ; Pseudomonas - enzymology ; Pseudomonas - genetics ; Pseudomonas putida ; Urocanase ; Urocanate Hydratase - genetics</subject><ispartof>FEBS letters, 1991-07, Vol.286 (1-2), p.55-57</ispartof><rights>1991</rights><rights>FEBS Letters 286 (1991) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516Y-fcd1dd54c9e4691867edfc257f9c795f4713c13c82d0ab0e7fa19739053837573</citedby><cites>FETCH-LOGICAL-c516Y-fcd1dd54c9e4691867edfc257f9c795f4713c13c82d0ab0e7fa19739053837573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001457939180938Y$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19858244$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1677899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fessenmaier, Martin</creatorcontrib><creatorcontrib>Frank, Rainer</creatorcontrib><creatorcontrib>Retey, János</creatorcontrib><creatorcontrib>Schubert, Carsten</creatorcontrib><title>Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60 771 Da.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>DNA, Bacterial</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Histidine - metabolism</subject><subject>hutU Gene</subject><subject>Lyases</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>PCR</subject><subject>Polymerase Chain Reaction</subject><subject>Pseudomonas - enzymology</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas putida</subject><subject>Urocanase</subject><subject>Urocanate Hydratase - genetics</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1LHTEUhkOp6FX7D1qYTUUXY5PJZJJsBHvxVkGwC13cVYjJiUZmJrfJjMV_b8a56K4UAuGc9z0feYLQV4JPCSbND4xJXTIu6bEkJwJLKsr1J7QggtOS1o34jBbvlj20n9ITzrEgchftkoZzIeUCXS7b0Pv-odC9LRL8GaE3Uzg8QjHGYHSvExQP0ENx_DgOdyeFi6ErficYbehCVovNOHirD9GO022CL9v7AN2tLm6Xl-X1za-r5fl1aRhp1qUzlljLaiOhbiQRDQfrTMW4k4ZL5mpOqMlHVBbrewzcaSI5lZhRQTnj9AAdzX03MeRt06A6nwy0re4hjEkJzCvSMJyN9Ww0MaQUwalN9J2OL4pgNQFUEx010VGSqDeAap3Lvm37j_cd2I-imVjWv291nYxuXdQZWPqwScFEVdfZt5p9f30LL_81W60uflaTMOUzmyk7LXQ2N4JM9dlDVMn4_EtgfQQzKBv8v1_0Clj3n2Y</recordid><startdate>19910729</startdate><enddate>19910729</enddate><creator>Fessenmaier, Martin</creator><creator>Frank, Rainer</creator><creator>Retey, János</creator><creator>Schubert, Carsten</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19910729</creationdate><title>Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida</title><author>Fessenmaier, Martin ; Frank, Rainer ; Retey, János ; Schubert, Carsten</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516Y-fcd1dd54c9e4691867edfc257f9c795f4713c13c82d0ab0e7fa19739053837573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>DNA, Bacterial</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Histidine - metabolism</topic><topic>hutU Gene</topic><topic>Lyases</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>PCR</topic><topic>Polymerase Chain Reaction</topic><topic>Pseudomonas - enzymology</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas putida</topic><topic>Urocanase</topic><topic>Urocanate Hydratase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fessenmaier, Martin</creatorcontrib><creatorcontrib>Frank, Rainer</creatorcontrib><creatorcontrib>Retey, János</creatorcontrib><creatorcontrib>Schubert, Carsten</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fessenmaier, Martin</au><au>Frank, Rainer</au><au>Retey, János</au><au>Schubert, Carsten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1991-07-29</date><risdate>1991</risdate><volume>286</volume><issue>1-2</issue><spage>55</spage><epage>57</epage><pages>55-57</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. 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| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1991-07, Vol.286 (1-2), p.55-57 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80721650 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Cloning, Molecular DNA, Bacterial Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Genes, Bacterial Histidine - metabolism hutU Gene Lyases Molecular Sequence Data Multigene Family PCR Polymerase Chain Reaction Pseudomonas - enzymology Pseudomonas - genetics Pseudomonas putida Urocanase Urocanate Hydratase - genetics |
| title | Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida |
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