Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida

A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60 771 Da.

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Bibliographische Detailangaben
Veröffentlicht in:	FEBS letters 1991-07, Vol.286 (1-2), p.55-57
Hauptverfasser:	Fessenmaier, Martin, Frank, Rainer, Retey, János, Schubert, Carsten
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Cloning, Molecular DNA, Bacterial Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Genes, Bacterial Histidine - metabolism hutU Gene Lyases Molecular Sequence Data Multigene Family PCR Polymerase Chain Reaction Pseudomonas - enzymology Pseudomonas - genetics Pseudomonas putida Urocanase Urocanate Hydratase - genetics
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Beschreibung
Zusammenfassung:	A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60 771 Da.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(91)80938-Y