Sequence and expression in Escherichia coli of a Mycoplasma hominis gene encoding elongation factor Tu

We describe the molecular cloning and the complete nucleotide (nt) sequence of a Mycoplasma hominis gene common to a broad range of Mycoplasma species, as defined by hybridization analysis with the cloned gene. Production of M. hominis protein in Escherichia coli was assayed by use of a monoclonal a...

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Veröffentlicht in:	Gene 1991-06, Vol.102 (1), p.123-127
Hauptverfasser:	Lüneberg, Edeltraud, Kamla, Volker, Hadding, Ulrich, Frosch, Matthias
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antibodies, Monoclonal Antigens, Bacterial - genetics Base Sequence Biological and medical sciences Blotting, Southern Blotting, Western Cloning, Molecular elongation factor EF-Tu Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology genes Genes. Genome Genetic Variation genomic library Molecular and cellular biology Molecular genetics Molecular Sequence Data monoclonal antibody Multigene Family - genetics Mycoplasma - genetics Mycoplasma hominis nucleotide sequence Peptide Elongation Factor Tu - biosynthesis Peptide Elongation Factor Tu - genetics Plasmids - genetics predictions Recombinant DNA Recombinant Proteins - biosynthesis Restriction Mapping Sequence Homology, Nucleic Acid
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Zusammenfassung:	We describe the molecular cloning and the complete nucleotide (nt) sequence of a Mycoplasma hominis gene common to a broad range of Mycoplasma species, as defined by hybridization analysis with the cloned gene. Production of M. hominis protein in Escherichia coli was assayed by use of a monoclonal antibody. The nt sequence analysis revealed a 1194-bp open reading frame that could encode a 43 516-Da protein. Computer-aided sequence comparison indicated that the gene codes for elongation factor Tu.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(91)90550-U