Comparison of calcium, freeze-thaw, and Triton X-100 tegumental disruption/recovery techniques applied to Schistosoma mansoni

Schistosoma mansoni, comparative evaluation of 3 methods for isolating tegument (use of Triton X-100, freezing and thawing, and high ionic strength calcium), morphological characteristics of isolate's contents, recovery of enzymatic activities commonly used as plasmalemmal markers, specific rec...

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Veröffentlicht in:	The Journal of parasitology 1983-06, Vol.69 (3), p.519-533
Hauptverfasser:	Oaks, J.A, Cain, G.D, Mower, D.A, Raj, R.K
Format:	Artikel
Sprache:	eng
Schlagworte:	5'-Nucleotidase Adenosine triphosphatases Adenosine Triphosphatases - metabolism Alkaline Phosphatase - metabolism Animals Basement membrane Calcium Calcium Chloride Canals Cell Fractionation - methods Cell Membrane - analysis Cell Membrane - ultrastructure Cell membranes Concanavalin A - analysis Cytoplasm Electron micrographs Enzymes Freezing Materials Membrane Proteins - analysis Membranes, Trematoda Microscopy, Electron Nucleotidases - metabolism Octoxynol Parasitology Polyethylene Glycols Schistosoma mansoni Schistosoma mansoni - analysis Schistosoma mansoni - ultrastructure Structure and Ultrastructure Technique, Specimen preparation and preservation Tegument Trematoda
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Beschreibung
Zusammenfassung:	Schistosoma mansoni, comparative evaluation of 3 methods for isolating tegument (use of Triton X-100, freezing and thawing, and high ionic strength calcium), morphological characteristics of isolate's contents, recovery of enzymatic activities commonly used as plasmalemmal markers, specific recovery of surface membrane-bound lectin tags, quantity of membrane recovered, condition of carcasses of treated worms
ISSN:	0022-3395 1937-2345
DOI:	10.2307/3281366