Specific cleavage of chicken alpha A-globin and human c-Ha-ras genes by two molecular forms of calf thymus topoisomerase I

Specific cleavage of chicken alpha A-globin and human c-Ha-ras genes by two molecular forms of calf thymus topoisomerase I

Two molecular forms of topoisomerase I differing in size and sensitivity to camptothecin were isolated from calf thymus. Mapping of topo I cleavage sites of the cloned chicken alpha A-globin and human c-Ha-ras genes was carried out. Camptothecin was shown to affect site specificity of the topoisomer...

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Veröffentlicht in:Molecular and cellular biochemistry 1991-03, Vol.101 (2), p.115-124
Hauptverfasser: Bronstein, I B, Gromova, I I, Razin, S V
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Base Sequence
Binding Sites
Camptothecin - metabolism
Cattle
Chickens
DNA - chemistry
DNA Topoisomerases, Type I - metabolism
Genes, ras
Globins - chemistry
Globins - genetics
Humans
Hydrolysis
Molecular Sequence Data
Peptide Mapping
Thymus Gland - enzymology
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Beschreibung
Zusammenfassung:Two molecular forms of topoisomerase I differing in size and sensitivity to camptothecin were isolated from calf thymus. Mapping of topo I cleavage sites of the cloned chicken alpha A-globin and human c-Ha-ras genes was carried out. Camptothecin was shown to affect site specificity of the topoisomerases.
ISSN:0300-8177
1573-4919
DOI:10.1007/BF00229529