Simultaneous determination of total cholesterol concentration and radioactivity in plasma
Total plama cholesterol concentration and radioactivity were measured simultaneously using a gas chromatograph equipped with a flame ionization detector and an effluent splitter. More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with...
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| Veröffentlicht in: | Journal of biochemical and biophysical methods 1983-01, Vol.8 (1), p.1-7 |
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| container_title | Journal of biochemical and biophysical methods |
| container_volume | 8 |
| creator | Emmanuel, B. Fenton, T.W. Turner, B.V. Milligan, L.P. |
| description | Total plama cholesterol concentration and radioactivity were measured simultaneously using a gas chromatograph equipped with a flame ionization detector and an effluent splitter. More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with the amounts of labeled cholesterol present in plasma. The recovery of label was quantitative over a wide range of carrier cholesterol concentration. The method is highly reproducible, accurate, rapid and specific. |
| doi_str_mv | 10.1016/0165-022X(83)90015-5 |
| format | Article |
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More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with the amounts of labeled cholesterol present in plasma. The recovery of label was quantitative over a wide range of carrier cholesterol concentration. The method is highly reproducible, accurate, rapid and specific.</description><identifier>ISSN: 0165-022X</identifier><identifier>EISSN: 1872-857X</identifier><identifier>DOI: 10.1016/0165-022X(83)90015-5</identifier><identifier>PMID: 6630864</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Acetates - metabolism ; Analytical, structural and metabolic biochemistry ; animal science ; Animals ; Biological and medical sciences ; Cholesterol - blood ; Chromatography, Gas - methods ; Fundamental and applied biological sciences. Psychology ; Liver - metabolism ; Other biological molecules ; plasma ; Propionates - metabolism ; radioacticity ; Rats ; Terpenes, steroids. 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More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with the amounts of labeled cholesterol present in plasma. The recovery of label was quantitative over a wide range of carrier cholesterol concentration. The method is highly reproducible, accurate, rapid and specific.</description><subject>Acetates - metabolism</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>animal science</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cholesterol - blood</subject><subject>Chromatography, Gas - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Liver - metabolism</subject><subject>Other biological molecules</subject><subject>plasma</subject><subject>Propionates - metabolism</subject><subject>radioacticity</subject><subject>Rats</subject><subject>Terpenes, steroids. Hormones</subject><subject>total cholesterol concentration</subject><issn>0165-022X</issn><issn>1872-857X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtr3DAURkVoSadJ_0FLvQilXTi9etjSbAIl9AWBLtJAuhJXDzcKtjWRNIH8-2riYZZdCAm-cx86hLylcE6B9p_r6Vpg7Paj4p_WALRruyOyokqyVnXy9gVZHZBX5HXO9wDAFRPH5LjvOaherMif6zBtx4Kzj9vcOF98msKMJcS5iUNTYsGxsXdx9LlGsb7jbP1c0oLg7JqELkS0JTyG8tSEudmMmCc8JS8HHLN_s79PyM23r78vf7RXv77_vPxy1VpBobTe2bUxneiYoRKwt9SiVz1IZ5FyyoyjBnop6MCltQMOQnDjDJeI3Bvu-An5sPTdpPiwrWvqKWTrx3H5k1YgKTCpKigW0KaYc_KD3qQwYXrSFPTOqN7p0jtdWnH9bFR3tezdvv_WTN4divYKa362zzFbHIeEsw35gK05FYL1FXu_YANGjX9TRW6uGVBe54FcS1mJi4Xw1dZj8ElnG3y17ULytmgXw_83_Qcs6J4L</recordid><startdate>19830101</startdate><enddate>19830101</enddate><creator>Emmanuel, B.</creator><creator>Fenton, T.W.</creator><creator>Turner, B.V.</creator><creator>Milligan, L.P.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19830101</creationdate><title>Simultaneous determination of total cholesterol concentration and radioactivity in plasma</title><author>Emmanuel, B. ; Fenton, T.W. ; Turner, B.V. ; Milligan, L.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-edc9bb5452b170a6c1cae8607dca1312bd1b06741f37ccfaf443bdb37aa3eb3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Acetates - metabolism</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>animal science</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cholesterol - blood</topic><topic>Chromatography, Gas - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Liver - metabolism</topic><topic>Other biological molecules</topic><topic>plasma</topic><topic>Propionates - metabolism</topic><topic>radioacticity</topic><topic>Rats</topic><topic>Terpenes, steroids. Hormones</topic><topic>total cholesterol concentration</topic><toplevel>online_resources</toplevel><creatorcontrib>Emmanuel, B.</creatorcontrib><creatorcontrib>Fenton, T.W.</creatorcontrib><creatorcontrib>Turner, B.V.</creatorcontrib><creatorcontrib>Milligan, L.P.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemical and biophysical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Emmanuel, B.</au><au>Fenton, T.W.</au><au>Turner, B.V.</au><au>Milligan, L.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous determination of total cholesterol concentration and radioactivity in plasma</atitle><jtitle>Journal of biochemical and biophysical methods</jtitle><addtitle>J Biochem Biophys Methods</addtitle><date>1983-01-01</date><risdate>1983</risdate><volume>8</volume><issue>1</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><issn>0165-022X</issn><eissn>1872-857X</eissn><abstract>Total plama cholesterol concentration and radioactivity were measured simultaneously using a gas chromatograph equipped with a flame ionization detector and an effluent splitter. More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with the amounts of labeled cholesterol present in plasma. The recovery of label was quantitative over a wide range of carrier cholesterol concentration. The method is highly reproducible, accurate, rapid and specific.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>6630864</pmid><doi>10.1016/0165-022X(83)90015-5</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-022X |
| ispartof | Journal of biochemical and biophysical methods, 1983-01, Vol.8 (1), p.1-7 |
| issn | 0165-022X 1872-857X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80710278 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Acetates - metabolism Analytical, structural and metabolic biochemistry animal science Animals Biological and medical sciences Cholesterol - blood Chromatography, Gas - methods Fundamental and applied biological sciences. Psychology Liver - metabolism Other biological molecules plasma Propionates - metabolism radioacticity Rats Terpenes, steroids. Hormones total cholesterol concentration |
| title | Simultaneous determination of total cholesterol concentration and radioactivity in plasma |
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