Simultaneous determination of total cholesterol concentration and radioactivity in plasma

Total plama cholesterol concentration and radioactivity were measured simultaneously using a gas chromatograph equipped with a flame ionization detector and an effluent splitter. More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with...

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Veröffentlicht in:	Journal of biochemical and biophysical methods 1983-01, Vol.8 (1), p.1-7
Hauptverfasser:	Emmanuel, B., Fenton, T.W., Turner, B.V., Milligan, L.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetates - metabolism Analytical, structural and metabolic biochemistry animal science Animals Biological and medical sciences Cholesterol - blood Chromatography, Gas - methods Fundamental and applied biological sciences. Psychology Liver - metabolism Other biological molecules plasma Propionates - metabolism radioacticity Rats Terpenes, steroids. Hormones total cholesterol concentration
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Beschreibung
Zusammenfassung:	Total plama cholesterol concentration and radioactivity were measured simultaneously using a gas chromatograph equipped with a flame ionization detector and an effluent splitter. More than 99% of the recovered radioactivity was in the cholesterol peak. Specific activities were highly correlated with the amounts of labeled cholesterol present in plasma. The recovery of label was quantitative over a wide range of carrier cholesterol concentration. The method is highly reproducible, accurate, rapid and specific.
ISSN:	0165-022X 1872-857X
DOI:	10.1016/0165-022X(83)90015-5