A compact three-dimensional crystal form of the large ribosomal subunit from Bacillus stearothermophilus

A new form of well-ordered three-dimensional crystals of intact 50 S ribosomal subunits from Bacillus stearothermophilus have been obtained. Electron micrographs of positively stained sections of these crystals revealed that the ribosomal particles are packed closely. The cell parameters have been d...

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Veröffentlicht in:	FEBS letters 1983-10, Vol.163 (1), p.69-72
Hauptverfasser:	Yonath, A., Piefke, J., Müssig, J., Gewitz, H.-S., Wittmann, H.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus stearothermophilus Biological and medical sciences Computed contoured image Crystalline structure Crystallization Electron microscopy Fundamental and applied biological sciences. Psychology Geobacillus stearothermophilus - metabolism Geobacillus stearothermophilus - ultrastructure Microscopy, Electron Molecular biophysics New crystal form Ribosomal particle Ribosomes - metabolism Ribosomes - ultrastructure Structure in molecular biology Three-dimensional crystal Unit cell dimension
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Zusammenfassung:	A new form of well-ordered three-dimensional crystals of intact 50 S ribosomal subunits from Bacillus stearothermophilus have been obtained. Electron micrographs of positively stained sections of these crystals revealed that the ribosomal particles are packed closely. The cell parameters have been determined. Representative electron micrographs and their computed contoured filtered images are shown.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(83)81165-X