Acute effect of neodymium yttrium aluminium garment laser on the cerebral cortical structure, blood-brain barrier, and pial vessel behaviour in the cat

Experimental brain lesions were created by Nd:YAG laser (wave length 1.06 microns) irradiation on the cerebral cortex of anaesthesized adult cats with 20 Watts impacts of 0.5, 1.0, 2.5, and 5.0 seconds exposure time through cranial windows. Histological changes, disruption of the blood-brain barrier (Evans blue extravasation) and pial vessel reaction (large vessels more than 100 microns and vessels smaller than 100 microns) were studied under constant PaCO2, blood pH, and mean arterial pressure. Histological changes of the lesions consisted of a zone of dense coagulation, a pale zone of homogeneous coagulation and an oedematous zone. Evans blue extravasation was uniformly seen extending from the histologically changed area into the surrounding tissue in all experiments. Pial arteries in the area with morphological changes showed pronounced dilatation (100.0 +/- 7.2%) and one third of these arteries were closed by thrombi. Pial arteries in the area of Evans blue extravasation but outside of histological changes also dilated (large arteries 60 +/- 4.1%, small arteries 77 +/- 5.9%). Pial arteries outside of the Evans blue extravasation were affected transiently and only in a very small zone: Within a distance of 200 microns from the Evans blue extravasation, large arteries initially dilated by 41 +/- 8.3%; small arteries dilated within 400 microns (42 +/- 3.7%). Within 4 minutes after irradiation arterial dilatation was again significantly reduced (p less than 0.01). It is concluded that no important vascular changes occur beyond the zones of histologically altered brain tissue.