A new system for ATP-metric immunoanalysis

Treatment of amino-group-containing antigens with adenosine-5′-trimetaphosphate results in their chemical modification by -pppA residues. An immunoanalytical system is proposed based upon competition of these ATP-labelled antigens with those of the sample for immobilized antibodies. Mild acidic trea...

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Veröffentlicht in:	FEBS letters 1983-10, Vol.162 (2), p.266-269
Hauptverfasser:	Grachev, M.A., Dobrikov, M.I., Knorre, V.D., Pressman, E.K., Roschke, V.V., Shishkin, G.V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - chemical synthesis Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry ATP Biological and medical sciences Bioluminescence Cyclic AMP - analogs & derivatives Fundamental and applied biological sciences. Psychology Humans Immunoanalysis Immunoassay - methods Luciferase Luciferases Luminescence Myoglobin Myoglobin - analysis Radioimmunoassay Thyroxine Thyroxine - analysis
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Beschreibung
Zusammenfassung:	Treatment of amino-group-containing antigens with adenosine-5′-trimetaphosphate results in their chemical modification by -pppA residues. An immunoanalytical system is proposed based upon competition of these ATP-labelled antigens with those of the sample for immobilized antibodies. Mild acidic treatment of complexes of ATP-labelled antigens with immobilized antibodies results in quantitative liberation of intact ATP. The latter may be determined by the ultrosensitive bioluminescent techniques based upon emission of light with firefly luciferase. The validity of the system has been studied with two clinically important antigens, thyroxine and myoglobin.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(83)80769-8