Purification of reduced nicotinamide adenine dinucleotide by ion-exchange and high-performance liquid chromatography

Combining ion-exchange (AG MP-1) and reversed-phase (C-18) partition chromatography accomplishes a higher degree of purification of NADH than either method can provide alone. Final elution in 95% ethanol, dehydration with anhydrous sodium sulfate, and storage in dry 1,2-propanediol over molecular si...

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Veröffentlicht in:	Analytical biochemistry 1983-07, Vol.132 (1), p.50-53
Hauptverfasser:	Newton, Colin J., Faynor, Steven M., Northrop, Dexter B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid Chromatography, Ion Exchange Fundamental and applied biological sciences. Psychology NAD - isolation & purification Nucleic acids Nucleic bases, nucleotides Oxidation-Reduction Polystyrenes
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Beschreibung
Zusammenfassung:	Combining ion-exchange (AG MP-1) and reversed-phase (C-18) partition chromatography accomplishes a higher degree of purification of NADH than either method can provide alone. Final elution in 95% ethanol, dehydration with anhydrous sodium sulfate, and storage in dry 1,2-propanediol over molecular sieves prevents hydrolysis of the purified dinucleotide.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(83)90424-4