Ultraviolet light induction of lambda from dcm host strains alleviates EcoRII restriction of phage

A new form of restriction alleviation is demonstrated for phage induced by ultraviolet light from dcm strains of Escherichia coli K-12. EcoRII restriction of the induced phage is alleviated, which is the first report of Type II restriction alleviation. Unlike previously reported restriction alleviat...

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Veröffentlicht in:	FEMS microbiology letters 1991-04, Vol.79 (2-3), p.121-125
Hauptverfasser:	RADNEDGE, L, PINNEY, R. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophage lambda - growth & development Biological and medical sciences Deoxyribonucleases, Type II Site-Specific - metabolism DNA-Cytosine Methylases - biosynthesis Enzyme Induction - radiation effects Escherichia coli - enzymology Escherichia coli - radiation effects Fundamental and applied biological sciences. Psychology Genetics Lysogeny Microbiology Ultraviolet Rays Virology Virus Activation - radiation effects
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Zusammenfassung:	A new form of restriction alleviation is demonstrated for phage induced by ultraviolet light from dcm strains of Escherichia coli K-12. EcoRII restriction of the induced phage is alleviated, which is the first report of Type II restriction alleviation. Unlike previously reported restriction alleviation, the increase in phage-plating efficiency is not dependent upon irradiation of the plating host for its induction.
ISSN:	0378-1097 1574-6968