Complement and antibody mediate enhancement of HIV infection by increasing virus binding and provirus formation

Previous studies have shown that infection of complement receptor (CR2)-bearing cells with HIV pretreated with antibody (Ab) plus complement (C) resulted in increased virus expression. The current study was designed to determine whether C-mediated 'enhancement' of HIV-1 production was the...

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Veröffentlicht in:AIDS (London) 1991-03, Vol.5 (3), p.269-274
Hauptverfasser: JUNE, R. A, SCHADE, S. Z, BANKOWSKI, M. J, KUHNS, M, MCNAMARA, A, LINT, T. F, LANDAY, A. L, SPEAR, G. T
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Sprache:eng
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Zusammenfassung:Previous studies have shown that infection of complement receptor (CR2)-bearing cells with HIV pretreated with antibody (Ab) plus complement (C) resulted in increased virus expression. The current study was designed to determine whether C-mediated 'enhancement' of HIV-1 production was the result of increased virus infection of cells as assessed by provirus formation and virus binding. Virus was incubated with anti-HIV Ab and/or C and added to CR2-positive MT-2 cells. Increased virus expression by MT-2 cells correlated with increased numbers of HIV-immunofluorescent-positive cells at 24 and 48 h and higher levels of provirus detected 8-28 h after infection. MT-2 cells also bound threefold more Ab-plus-C-treated virus than untreated virus. Serial dilutions of C showed that high levels of C with Ab did not enhance but rather suppressed virus expression. Studies were also performed which showed that terminal C components C5 and C8 were not necessary for the enhancing effect. The increased binding of C-coated HIV to CR-positive cells has important implications for the fate of virus in vivo.
ISSN:0269-9370
1473-5571
DOI:10.1097/00002030-199103000-00004