Antithrombin III synthesis in rat liver parenchymal cells
We have previously demonstrated by immunoperoxydase the presence of immunoreactive antithrombin III (AT III) in rat hepatocytes. We now present direct evidence that rat hepatocytes in culture synthesize AT III like immunoreactive material : 35S-methionine was added to the culture medium and incubate...
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| Veröffentlicht in: | Thrombosis research 1983-05, Vol.30 (4), p.369-375 |
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| container_end_page | 375 |
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| container_issue | 4 |
| container_start_page | 369 |
| container_title | Thrombosis research |
| container_volume | 30 |
| creator | Léon, M. Aiach, M. Coezy, E. Guennec, J.-Y. Fiessinger, J.-N. |
| description | We have previously demonstrated by immunoperoxydase the presence of immunoreactive antithrombin III (AT III) in rat hepatocytes. We now present direct evidence that rat hepatocytes in culture synthesize AT III like immunoreactive material :
35S-methionine was added to the culture medium and incubated with hepatocytes. After incubation, AT III was immunologically characterized in the medium. We found significant amounts of
35S-AT III among the radioactive proteins synthesized and secreted by the cells. |
| doi_str_mv | 10.1016/0049-3848(83)90228-1 |
| format | Article |
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35S-methionine was added to the culture medium and incubated with hepatocytes. After incubation, AT III was immunologically characterized in the medium. We found significant amounts of
35S-AT III among the radioactive proteins synthesized and secreted by the cells.</description><identifier>ISSN: 0049-3848</identifier><identifier>EISSN: 1879-2472</identifier><identifier>DOI: 10.1016/0049-3848(83)90228-1</identifier><identifier>PMID: 6612676</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; Antithrombin III ; Antithrombin III - biosynthesis ; Antithrombin III - immunology ; Antithrombin III synthesis ; Cells, Cultured ; Liver - metabolism ; Male ; Methionine - metabolism ; Protein Biosynthesis ; Rat hepatocytes ; Rats ; Rats, Inbred Strains</subject><ispartof>Thrombosis research, 1983-05, Vol.30 (4), p.369-375</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-49acdf49bacaa8311e2db2732d0f44c1bd175eaf89d53c28b206ec4f93210fb33</citedby><cites>FETCH-LOGICAL-c272t-49acdf49bacaa8311e2db2732d0f44c1bd175eaf89d53c28b206ec4f93210fb33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0049-3848(83)90228-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6612676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Léon, M.</creatorcontrib><creatorcontrib>Aiach, M.</creatorcontrib><creatorcontrib>Coezy, E.</creatorcontrib><creatorcontrib>Guennec, J.-Y.</creatorcontrib><creatorcontrib>Fiessinger, J.-N.</creatorcontrib><title>Antithrombin III synthesis in rat liver parenchymal cells</title><title>Thrombosis research</title><addtitle>Thromb Res</addtitle><description>We have previously demonstrated by immunoperoxydase the presence of immunoreactive antithrombin III (AT III) in rat hepatocytes. We now present direct evidence that rat hepatocytes in culture synthesize AT III like immunoreactive material :
35S-methionine was added to the culture medium and incubated with hepatocytes. After incubation, AT III was immunologically characterized in the medium. We found significant amounts of
35S-AT III among the radioactive proteins synthesized and secreted by the cells.</description><subject>Animals</subject><subject>Antithrombin III</subject><subject>Antithrombin III - biosynthesis</subject><subject>Antithrombin III - immunology</subject><subject>Antithrombin III synthesis</subject><subject>Cells, Cultured</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Methionine - metabolism</subject><subject>Protein Biosynthesis</subject><subject>Rat hepatocytes</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><issn>0049-3848</issn><issn>1879-2472</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcKeRA-rmSTdTS5CKX4sFLzoOWSTWRrZj5psC_337tri0dPAvO-8M_MQcg30AShkj5QKlXIp5J3k94oyJlM4IVOQuUqZyNkpmf5ZzslFjF-UQg5qPiGTLAOW5dmUqEXb-34duqb0bVIURRL3bb_G6GMyNILpk9rvMCQbE7C1631j6sRiXcdLclaZOuLVsc7I58vzx_ItXb2_FsvFKrUsZ30qlLGuEqo01hjJAZC5kuWcOVoJYaF0kM_RVFK5ObdMloxmaEWlOANalZzPyO0hdxO67y3GXjc-jheYFrtt1JJmYvhlNIqD0YYuxoCV3gTfmLDXQPVITI849IhDS65_iWkYxm6O-duyQfc3dEQ06E8HHYcndx6DjtYPKND5gLbXrvP_L_gBZvl6Rw</recordid><startdate>19830515</startdate><enddate>19830515</enddate><creator>Léon, M.</creator><creator>Aiach, M.</creator><creator>Coezy, E.</creator><creator>Guennec, J.-Y.</creator><creator>Fiessinger, J.-N.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19830515</creationdate><title>Antithrombin III synthesis in rat liver parenchymal cells</title><author>Léon, M. ; Aiach, M. ; Coezy, E. ; Guennec, J.-Y. ; Fiessinger, J.-N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-49acdf49bacaa8311e2db2732d0f44c1bd175eaf89d53c28b206ec4f93210fb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Antithrombin III</topic><topic>Antithrombin III - biosynthesis</topic><topic>Antithrombin III - immunology</topic><topic>Antithrombin III synthesis</topic><topic>Cells, Cultured</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Methionine - metabolism</topic><topic>Protein Biosynthesis</topic><topic>Rat hepatocytes</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Léon, M.</creatorcontrib><creatorcontrib>Aiach, M.</creatorcontrib><creatorcontrib>Coezy, E.</creatorcontrib><creatorcontrib>Guennec, J.-Y.</creatorcontrib><creatorcontrib>Fiessinger, J.-N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Thrombosis research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Léon, M.</au><au>Aiach, M.</au><au>Coezy, E.</au><au>Guennec, J.-Y.</au><au>Fiessinger, J.-N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antithrombin III synthesis in rat liver parenchymal cells</atitle><jtitle>Thrombosis research</jtitle><addtitle>Thromb Res</addtitle><date>1983-05-15</date><risdate>1983</risdate><volume>30</volume><issue>4</issue><spage>369</spage><epage>375</epage><pages>369-375</pages><issn>0049-3848</issn><eissn>1879-2472</eissn><abstract>We have previously demonstrated by immunoperoxydase the presence of immunoreactive antithrombin III (AT III) in rat hepatocytes. We now present direct evidence that rat hepatocytes in culture synthesize AT III like immunoreactive material :
35S-methionine was added to the culture medium and incubated with hepatocytes. After incubation, AT III was immunologically characterized in the medium. We found significant amounts of
35S-AT III among the radioactive proteins synthesized and secreted by the cells.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>6612676</pmid><doi>10.1016/0049-3848(83)90228-1</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0049-3848 |
| ispartof | Thrombosis research, 1983-05, Vol.30 (4), p.369-375 |
| issn | 0049-3848 1879-2472 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Antithrombin III Antithrombin III - biosynthesis Antithrombin III - immunology Antithrombin III synthesis Cells, Cultured Liver - metabolism Male Methionine - metabolism Protein Biosynthesis Rat hepatocytes Rats Rats, Inbred Strains |
| title | Antithrombin III synthesis in rat liver parenchymal cells |
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