Antithrombin III synthesis in rat liver parenchymal cells

Antithrombin III synthesis in rat liver parenchymal cells

We have previously demonstrated by immunoperoxydase the presence of immunoreactive antithrombin III (AT III) in rat hepatocytes. We now present direct evidence that rat hepatocytes in culture synthesize AT III like immunoreactive material : 35S-methionine was added to the culture medium and incubate...

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Veröffentlicht in:Thrombosis research 1983-05, Vol.30 (4), p.369-375
Hauptverfasser: Léon, M., Aiach, M., Coezy, E., Guennec, J.-Y., Fiessinger, J.-N.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antithrombin III
Antithrombin III - biosynthesis
Antithrombin III - immunology
Antithrombin III synthesis
Cells, Cultured
Liver - metabolism
Male
Methionine - metabolism
Protein Biosynthesis
Rat hepatocytes
Rats
Rats, Inbred Strains
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Zusammenfassung:We have previously demonstrated by immunoperoxydase the presence of immunoreactive antithrombin III (AT III) in rat hepatocytes. We now present direct evidence that rat hepatocytes in culture synthesize AT III like immunoreactive material : 35S-methionine was added to the culture medium and incubated with hepatocytes. After incubation, AT III was immunologically characterized in the medium. We found significant amounts of 35S-AT III among the radioactive proteins synthesized and secreted by the cells.
ISSN:0049-3848
1879-2472
DOI:10.1016/0049-3848(83)90228-1