Estradiol binding activity in epididymal cytosol of the turtle, Chrysemys picta
Estrogen binding activity was investigated in the epididymis of the turtle, Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity ( K d :8.0 × 10 −10 M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididym...
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| Veröffentlicht in: | General and comparative endocrinology 1983-07, Vol.51 (1), p.61-65 |
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| container_title | General and comparative endocrinology |
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| creator | Dufaure, Jean-Pierre Mak, Paul Callard, Ian P. |
| description | Estrogen binding activity was investigated in the epididymis of the turtle,
Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity (
K
d
:8.0 × 10
−10
M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididymal cytosol. In addition, binding of estradiol-17β was sensitive to excess (100-fold) diethylstilbestrol or natural estrogens (estradiol-17β, estrone, and estriol) but not to progesterone or androgens (testosterone and 5α-dihydrotestosterone). The specific estrogen binding macromolecules eluted from DNA-cellulose columns sedimented at 4–5 S in linear 5–20% sucrose gradients. These characteristics suggest the presence of an estrogen receptor in this androgen target organ. |
| doi_str_mv | 10.1016/0016-6480(83)90097-7 |
| format | Article |
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Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity (
K
d
:8.0 × 10
−10
M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididymal cytosol. In addition, binding of estradiol-17β was sensitive to excess (100-fold) diethylstilbestrol or natural estrogens (estradiol-17β, estrone, and estriol) but not to progesterone or androgens (testosterone and 5α-dihydrotestosterone). The specific estrogen binding macromolecules eluted from DNA-cellulose columns sedimented at 4–5 S in linear 5–20% sucrose gradients. These characteristics suggest the presence of an estrogen receptor in this androgen target organ.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1016/0016-6480(83)90097-7</identifier><identifier>PMID: 6884762</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Binding, Competitive ; Chromatography, Affinity ; Cytosol - metabolism ; Epididymis - metabolism ; Estradiol - metabolism ; Male ; Turtles - metabolism</subject><ispartof>General and comparative endocrinology, 1983-07, Vol.51 (1), p.61-65</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-4df8c6ff0874b42cc90c40490643e6b736e998c65aa64f98754f3d63a4b06b233</citedby><cites>FETCH-LOGICAL-c357t-4df8c6ff0874b42cc90c40490643e6b736e998c65aa64f98754f3d63a4b06b233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0016648083900977$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6884762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dufaure, Jean-Pierre</creatorcontrib><creatorcontrib>Mak, Paul</creatorcontrib><creatorcontrib>Callard, Ian P.</creatorcontrib><title>Estradiol binding activity in epididymal cytosol of the turtle, Chrysemys picta</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>Estrogen binding activity was investigated in the epididymis of the turtle,
Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity (
K
d
:8.0 × 10
−10
M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididymal cytosol. In addition, binding of estradiol-17β was sensitive to excess (100-fold) diethylstilbestrol or natural estrogens (estradiol-17β, estrone, and estriol) but not to progesterone or androgens (testosterone and 5α-dihydrotestosterone). The specific estrogen binding macromolecules eluted from DNA-cellulose columns sedimented at 4–5 S in linear 5–20% sucrose gradients. These characteristics suggest the presence of an estrogen receptor in this androgen target organ.</description><subject>Animals</subject><subject>Binding, Competitive</subject><subject>Chromatography, Affinity</subject><subject>Cytosol - metabolism</subject><subject>Epididymis - metabolism</subject><subject>Estradiol - metabolism</subject><subject>Male</subject><subject>Turtles - metabolism</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMoWj_-gUJOouDqbJPNJhdBSv0AoRc9h2wyq5H9qEla2H_v1haPXmYO87wzzEPIeQ63OeTiDsaSCS7hSrJrBaDKrNwjkxxUkQnJYZ9M_pAjchzjFwAUTOSH5FBIyUsxnZDFPKZgnO8bWvnO-e6DGpv82qeB-o7i0jvvhtY01A6pjyPW1zR9Ik2rkBq8obPPMERsh0iX3iZzSg5q00Q82_UT8v44f5s9Z6-Lp5fZw2tmWVGmjLtaWlHXIEte8am1CiwHrkBwhqIqmUClRqIwRvBaybLgNXOCGV6BqKaMnZDL7d5l6L9XGJNufbTYNKbDfhW1BDHNVSFGkG9BG_oYA9Z6GXxrwqBz0BuPeiNJbyRpyfSvR12OsYvd_lXVovsL7cSN8_vtHMcn1x6DjtZjZ9H5gDZp1_v_D_wArDGBpw</recordid><startdate>198307</startdate><enddate>198307</enddate><creator>Dufaure, Jean-Pierre</creator><creator>Mak, Paul</creator><creator>Callard, Ian P.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198307</creationdate><title>Estradiol binding activity in epididymal cytosol of the turtle, Chrysemys picta</title><author>Dufaure, Jean-Pierre ; Mak, Paul ; Callard, Ian P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-4df8c6ff0874b42cc90c40490643e6b736e998c65aa64f98754f3d63a4b06b233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Animals</topic><topic>Binding, Competitive</topic><topic>Chromatography, Affinity</topic><topic>Cytosol - metabolism</topic><topic>Epididymis - metabolism</topic><topic>Estradiol - metabolism</topic><topic>Male</topic><topic>Turtles - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dufaure, Jean-Pierre</creatorcontrib><creatorcontrib>Mak, Paul</creatorcontrib><creatorcontrib>Callard, Ian P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dufaure, Jean-Pierre</au><au>Mak, Paul</au><au>Callard, Ian P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Estradiol binding activity in epididymal cytosol of the turtle, Chrysemys picta</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>1983-07</date><risdate>1983</risdate><volume>51</volume><issue>1</issue><spage>61</spage><epage>65</epage><pages>61-65</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><abstract>Estrogen binding activity was investigated in the epididymis of the turtle,
Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity (
K
d
:8.0 × 10
−10
M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididymal cytosol. In addition, binding of estradiol-17β was sensitive to excess (100-fold) diethylstilbestrol or natural estrogens (estradiol-17β, estrone, and estriol) but not to progesterone or androgens (testosterone and 5α-dihydrotestosterone). The specific estrogen binding macromolecules eluted from DNA-cellulose columns sedimented at 4–5 S in linear 5–20% sucrose gradients. These characteristics suggest the presence of an estrogen receptor in this androgen target organ.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6884762</pmid><doi>10.1016/0016-6480(83)90097-7</doi><tpages>5</tpages></addata></record> |
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| ispartof | General and comparative endocrinology, 1983-07, Vol.51 (1), p.61-65 |
| issn | 0016-6480 1095-6840 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Binding, Competitive Chromatography, Affinity Cytosol - metabolism Epididymis - metabolism Estradiol - metabolism Male Turtles - metabolism |
| title | Estradiol binding activity in epididymal cytosol of the turtle, Chrysemys picta |
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