Estradiol binding activity in epididymal cytosol of the turtle, Chrysemys picta
Estrogen binding activity was investigated in the epididymis of the turtle, Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity ( K d :8.0 × 10 −10 M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididym...
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Veröffentlicht in: | General and comparative endocrinology 1983-07, Vol.51 (1), p.61-65 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Estrogen binding activity was investigated in the epididymis of the turtle,
Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity (
K
d
:8.0 × 10
−10
M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididymal cytosol. In addition, binding of estradiol-17β was sensitive to excess (100-fold) diethylstilbestrol or natural estrogens (estradiol-17β, estrone, and estriol) but not to progesterone or androgens (testosterone and 5α-dihydrotestosterone). The specific estrogen binding macromolecules eluted from DNA-cellulose columns sedimented at 4–5 S in linear 5–20% sucrose gradients. These characteristics suggest the presence of an estrogen receptor in this androgen target organ. |
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ISSN: | 0016-6480 1095-6840 |
DOI: | 10.1016/0016-6480(83)90097-7 |