Estradiol binding activity in epididymal cytosol of the turtle, Chrysemys picta

Estrogen binding activity was investigated in the epididymis of the turtle, Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity ( K d :8.0 × 10 −10 M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididym...

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Veröffentlicht in:General and comparative endocrinology 1983-07, Vol.51 (1), p.61-65
Hauptverfasser: Dufaure, Jean-Pierre, Mak, Paul, Callard, Ian P.
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Sprache:eng
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Zusammenfassung:Estrogen binding activity was investigated in the epididymis of the turtle, Chrysemys picta using DNA-cellulose affinity chromatography. A component binding estradiol-17β specifically with high affinity ( K d :8.0 × 10 −10 M) and limited capacity (20 fmol/mg protein) was demonstrated in the epididymal cytosol. In addition, binding of estradiol-17β was sensitive to excess (100-fold) diethylstilbestrol or natural estrogens (estradiol-17β, estrone, and estriol) but not to progesterone or androgens (testosterone and 5α-dihydrotestosterone). The specific estrogen binding macromolecules eluted from DNA-cellulose columns sedimented at 4–5 S in linear 5–20% sucrose gradients. These characteristics suggest the presence of an estrogen receptor in this androgen target organ.
ISSN:0016-6480
1095-6840
DOI:10.1016/0016-6480(83)90097-7