Positively and negatively acting signals regulating stalk cell and anterior-like cell differentiation in dictyostelium
The Dictyostelium ecmB gene encodes an extracellular matrix protein and is inducible by the stalk cell morphogen DIF. It is expressed in a subset of prestalk (pstB) cells in the slug and surrounding pstA cells first express it at culmination. A region of the ecmB promoter can direct transcription in...
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Veröffentlicht in: | Cell 1991-06, Vol.65 (6), p.983-989 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The Dictyostelium
ecmB gene encodes an extracellular matrix protein and is inducible by the stalk cell morphogen DIF. It is expressed in a subset of prestalk (pstB) cells in the slug and surrounding pstA cells first express it at culmination. A region of the
ecmB promoter can direct transcription in all anterior prestalk cells, but a separate, downstream region acts to prevent its expression in pstA cells prior to culmination. This may be the site of interaction of a repressor, regulated by an extracellular antagonist to DIF. At culmination, expression of the
ecmB gene also becomes greatly elevated in anterior-like cells as they move to surround the spore mass. A distal region of the
ecmB promoter directs increased expression in those anterior-like cells that surmount the spore head. This divergence in gene expression suggests that anterior-like cells and anterior prestalk cells experience different inductive conditions at culmination. |
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ISSN: | 0092-8674 1097-4172 |
DOI: | 10.1016/0092-8674(91)90550-I |