Supraoptic neurons in the rat hypothalamo-neurohypophysial explant: Double-labeling with lucifer yellow of injection and immunocytochemical identification of vasopressin- and neurophysin-containing neuroendocrine cells

By combining intracellular electrophysiology with double-labeled intracellular dye-marking and immunocytochemical identification of the same magnocellular neuroendocrine cell, we studied supraoptic neurons in the rat hypothalamo-neurohypophysial explant in vitro. This report examines neurophysiologi...

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Veröffentlicht in:Neuroscience letters 1983-06, Vol.37 (2), p.137-142
Hauptverfasser: Reaves, T.A., Hou-Yu, A., Zimmerman, E.A., Hayward, J.N.
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container_issue 2
container_start_page 137
container_title Neuroscience letters
container_volume 37
creator Reaves, T.A.
Hou-Yu, A.
Zimmerman, E.A.
Hayward, J.N.
description By combining intracellular electrophysiology with double-labeled intracellular dye-marking and immunocytochemical identification of the same magnocellular neuroendocrine cell, we studied supraoptic neurons in the rat hypothalamo-neurohypophysial explant in vitro. This report examines neurophysiological and light microscopical features of vasopressin- and neurophysin-immunoreactive, pituitary-projecting supraoptic neurons.
doi_str_mv 10.1016/0304-3940(83)90143-X
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ispartof Neuroscience letters, 1983-06, Vol.37 (2), p.137-142
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source MEDLINE; ScienceDirect Freedom Collection (Elsevier)
subjects Animals
Arginine Vasopressin - metabolism
Fluorescent Antibody Technique
Hypothalamo-Hypophyseal System - cytology
Male
Neurons - ultrastructure
Neurophysins - metabolism
Rats
Rats, Inbred Strains
Supraoptic Nucleus - cytology
title Supraoptic neurons in the rat hypothalamo-neurohypophysial explant: Double-labeling with lucifer yellow of injection and immunocytochemical identification of vasopressin- and neurophysin-containing neuroendocrine cells
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