Supraoptic neurons in the rat hypothalamo-neurohypophysial explant: Double-labeling with lucifer yellow of injection and immunocytochemical identification of vasopressin- and neurophysin-containing neuroendocrine cells

By combining intracellular electrophysiology with double-labeled intracellular dye-marking and immunocytochemical identification of the same magnocellular neuroendocrine cell, we studied supraoptic neurons in the rat hypothalamo-neurohypophysial explant in vitro. This report examines neurophysiological and light microscopical features of vasopressin- and neurophysin-immunoreactive, pituitary-projecting supraoptic neurons.