Picomole quantitation of ammonia by flow-through fluorometry

Picomole quantitation of ammonia by flow-through fluorometry

A new fiber optic fluorometer has been used to measure picomole amounts of ammonia. The method is based on the enzymatic conversion of α-ketoglutaric acid and ammonium ion to glutamate with associated oxidation of NADH. The decrease in NADH fluorescence is used to quantify sample ammonia content. Th...

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Veröffentlicht in:Analytical biochemistry 1983-04, Vol.130 (1), p.199-202
Hauptverfasser: Good, David W., Vurek, Gerald G.
Format: Artikel
Sprache:eng
Schlagworte:
Ammonia - isolation & purification
Chemical Phenomena
Chemistry
Fluorometry - instrumentation
Microchemistry
NAD
Oxidation-Reduction
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Zusammenfassung:A new fiber optic fluorometer has been used to measure picomole amounts of ammonia. The method is based on the enzymatic conversion of α-ketoglutaric acid and ammonium ion to glutamate with associated oxidation of NADH. The decrease in NADH fluorescence is used to quantify sample ammonia content. The method is rapid and reproducible and is capable of resolving differences as small as 0.3 pmol between samples containing 0–7 pmol.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(83)90670-X