Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining

Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphol...

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Veröffentlicht in:	Cytotechnology (Dordrecht) 1991-02, Vol.5 (2), p.141-146
Hauptverfasser:	NIKOLAI, T. J, PESHWA, M. V, GOETGHEBEUR, S, WEI-SHOU HU
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Animals Biological and medical sciences Biotechnology Cell cultures. Hybridization. Fusion Cell Division Cell Line - cytology Cell Survival Cricetinae Ethidium Fluoresceins Fluorescent Dyes Fundamental and applied biological sciences. Psychology Microspheres Molecular and cellular biology Vero Cells
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Beschreibung
Zusammenfassung:	Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.
ISSN:	0920-9069 1573-0778
DOI:	10.1007/BF00365430