Minor Histone 2A Variants and Ubiquinated Forms in the Native H2A:H2B Dimer
Histone octamers from calf thymus were separated into (H3:H4)$_{2}$ tetramers and H2A:H2B dimers by chromatography through Sephadex G100. The tetramers and dimers were analyzed for variants, ubiquitin adducts, and proteolyzed forms. The minor histone variants H2A.X and H2A.Z were found to be associa...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1983-07, Vol.221 (4609), p.468-470 |
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| container_issue | 4609 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Hatch, Christopher L. Bonner, William M. Moudrianakis, Evangelos N. |
| description | Histone octamers from calf thymus were separated into (H3:H4)$_{2}$ tetramers and H2A:H2B dimers by chromatography through Sephadex G100. The tetramers and dimers were analyzed for variants, ubiquitin adducts, and proteolyzed forms. The minor histone variants H2A.X and H2A.Z were found to be associated with histone H2B as H2A.X:H2B and H2A.Z:H2B dimers, respectively. Ubiquitin adducts of the H2A's and H2B were also present in H2A:H2B dimers. |
| doi_str_mv | 10.1126/science.6306766 |
| format | Article |
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The tetramers and dimers were analyzed for variants, ubiquitin adducts, and proteolyzed forms. The minor histone variants H2A.X and H2A.Z were found to be associated with histone H2B as H2A.X:H2B and H2A.Z:H2B dimers, respectively. Ubiquitin adducts of the H2A's and H2B were also present in H2A:H2B dimers.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.6306766</identifier><identifier>PMID: 6306766</identifier><language>eng</language><publisher>United States: The American Association for the Advancement of Science</publisher><subject>Adducts ; Animals ; calf thymus ; Cattle ; Cell research ; Chromatin ; Chromatography ; Chromatography, Gel ; Chromosomal Proteins, Non-Histone - metabolism ; Cytological research ; Dimers ; DNA ; Electrophoresis ; Electrophoresis, Polyacrylamide Gel ; gel chromatography ; Gels ; Histology ; Histones ; Histones - isolation & purification ; Histones - metabolism ; Molecular biology ; Nucleosomes ; Observations ; octamers ; ubiquitin ; Ubiquitins</subject><ispartof>Science (American Association for the Advancement of Science), 1983-07, Vol.221 (4609), p.468-470</ispartof><rights>Copyright 1983 The American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-46ae70ec5c47a4bf5bb527d8f625439ee9e699658c99fd2aa19e376b3e8409643</citedby><cites>FETCH-LOGICAL-c410t-46ae70ec5c47a4bf5bb527d8f625439ee9e699658c99fd2aa19e376b3e8409643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1690953$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1690953$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6306766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hatch, Christopher L.</creatorcontrib><creatorcontrib>Bonner, William M.</creatorcontrib><creatorcontrib>Moudrianakis, Evangelos N.</creatorcontrib><title>Minor Histone 2A Variants and Ubiquinated Forms in the Native H2A:H2B Dimer</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Histone octamers from calf thymus were separated into (H3:H4)$_{2}$ tetramers and H2A:H2B dimers by chromatography through Sephadex G100. The tetramers and dimers were analyzed for variants, ubiquitin adducts, and proteolyzed forms. The minor histone variants H2A.X and H2A.Z were found to be associated with histone H2B as H2A.X:H2B and H2A.Z:H2B dimers, respectively. Ubiquitin adducts of the H2A's and H2B were also present in H2A:H2B dimers.</description><subject>Adducts</subject><subject>Animals</subject><subject>calf thymus</subject><subject>Cattle</subject><subject>Cell research</subject><subject>Chromatin</subject><subject>Chromatography</subject><subject>Chromatography, Gel</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>Cytological research</subject><subject>Dimers</subject><subject>DNA</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>gel chromatography</subject><subject>Gels</subject><subject>Histology</subject><subject>Histones</subject><subject>Histones - isolation & purification</subject><subject>Histones - metabolism</subject><subject>Molecular biology</subject><subject>Nucleosomes</subject><subject>Observations</subject><subject>octamers</subject><subject>ubiquitin</subject><subject>Ubiquitins</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtPwzAUhS0EgvKYWUDyxILSOnbsxGylPIp4LcBqOc4NGCVOsV0E_55AIxiZ7nC-e3R0DkL7KRmnKRWTYCw4A2PBiMiFWEOjlEieSErYOhoRwkRSkJxvoe0QXgnpNck20eaAj9D1rXWdx3MbYucA0yl-0t5qFwPWrsKPpX1bWqcjVPii823A1uH4AvhOR_sOeE6nJ3N6is9sC34XbdS6CbA33B30eHH-MJsnN_eXV7PpTWKylMQkExpyAoabLNdZWfOy5DSvilpQnjEJIEFIKXhhpKwrqnUqgeWiZFBkRIqM7aCjle_Cd29LCFG1NhhoGu2gWwZVEJ5xnvN_wZT1dcgf8HgFPusGlHWmcxE-oumaBp5B9eln92pKC15QUvT0ZEUb34XgoVYLb1vtP1VK1PcqalhFDTX3H4dDkGXZQvXL_-kHK_21n8H_2QnZD8bYF8HSj9I</recordid><startdate>19830729</startdate><enddate>19830729</enddate><creator>Hatch, Christopher L.</creator><creator>Bonner, William M.</creator><creator>Moudrianakis, Evangelos N.</creator><general>The American Association for the Advancement of Science</general><general>American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19830729</creationdate><title>Minor Histone 2A Variants and Ubiquinated Forms in the Native H2A:H2B Dimer</title><author>Hatch, Christopher L. ; Bonner, William M. ; Moudrianakis, Evangelos N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-46ae70ec5c47a4bf5bb527d8f625439ee9e699658c99fd2aa19e376b3e8409643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Adducts</topic><topic>Animals</topic><topic>calf thymus</topic><topic>Cattle</topic><topic>Cell research</topic><topic>Chromatin</topic><topic>Chromatography</topic><topic>Chromatography, Gel</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>Cytological research</topic><topic>Dimers</topic><topic>DNA</topic><topic>Electrophoresis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>gel chromatography</topic><topic>Gels</topic><topic>Histology</topic><topic>Histones</topic><topic>Histones - isolation & purification</topic><topic>Histones - metabolism</topic><topic>Molecular biology</topic><topic>Nucleosomes</topic><topic>Observations</topic><topic>octamers</topic><topic>ubiquitin</topic><topic>Ubiquitins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hatch, Christopher L.</creatorcontrib><creatorcontrib>Bonner, William M.</creatorcontrib><creatorcontrib>Moudrianakis, Evangelos N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hatch, Christopher L.</au><au>Bonner, William M.</au><au>Moudrianakis, Evangelos N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Minor Histone 2A Variants and Ubiquinated Forms in the Native H2A:H2B Dimer</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1983-07-29</date><risdate>1983</risdate><volume>221</volume><issue>4609</issue><spage>468</spage><epage>470</epage><pages>468-470</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Histone octamers from calf thymus were separated into (H3:H4)$_{2}$ tetramers and H2A:H2B dimers by chromatography through Sephadex G100. The tetramers and dimers were analyzed for variants, ubiquitin adducts, and proteolyzed forms. The minor histone variants H2A.X and H2A.Z were found to be associated with histone H2B as H2A.X:H2B and H2A.Z:H2B dimers, respectively. Ubiquitin adducts of the H2A's and H2B were also present in H2A:H2B dimers.</abstract><cop>United States</cop><pub>The American Association for the Advancement of Science</pub><pmid>6306766</pmid><doi>10.1126/science.6306766</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1983-07, Vol.221 (4609), p.468-470 |
| issn | 0036-8075 1095-9203 |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Adducts Animals calf thymus Cattle Cell research Chromatin Chromatography Chromatography, Gel Chromosomal Proteins, Non-Histone - metabolism Cytological research Dimers DNA Electrophoresis Electrophoresis, Polyacrylamide Gel gel chromatography Gels Histology Histones Histones - isolation & purification Histones - metabolism Molecular biology Nucleosomes Observations octamers ubiquitin Ubiquitins |
| title | Minor Histone 2A Variants and Ubiquinated Forms in the Native H2A:H2B Dimer |
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