Minor Histone 2A Variants and Ubiquinated Forms in the Native H2A:H2B Dimer

Minor Histone 2A Variants and Ubiquinated Forms in the Native H2A:H2B Dimer

Histone octamers from calf thymus were separated into (H3:H4)$_{2}$ tetramers and H2A:H2B dimers by chromatography through Sephadex G100. The tetramers and dimers were analyzed for variants, ubiquitin adducts, and proteolyzed forms. The minor histone variants H2A.X and H2A.Z were found to be associa...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1983-07, Vol.221 (4609), p.468-470
Hauptverfasser: Hatch, Christopher L., Bonner, William M., Moudrianakis, Evangelos N.
Format: Artikel
Sprache:eng
Schlagworte:
Adducts
Animals
calf thymus
Cattle
Cell research
Chromatin
Chromatography
Chromatography, Gel
Chromosomal Proteins, Non-Histone - metabolism
Cytological research
Dimers
DNA
Electrophoresis
Electrophoresis, Polyacrylamide Gel
gel chromatography
Gels
Histology
Histones
Histones - isolation & purification
Histones - metabolism
Molecular biology
Nucleosomes
Observations
octamers
ubiquitin
Ubiquitins
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Zusammenfassung:Histone octamers from calf thymus were separated into (H3:H4)$_{2}$ tetramers and H2A:H2B dimers by chromatography through Sephadex G100. The tetramers and dimers were analyzed for variants, ubiquitin adducts, and proteolyzed forms. The minor histone variants H2A.X and H2A.Z were found to be associated with histone H2B as H2A.X:H2B and H2A.Z:H2B dimers, respectively. Ubiquitin adducts of the H2A's and H2B were also present in H2A:H2B dimers.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.6306766