The infectivity of herpes simplex virus dna in rat embryo cells is enhanced synergistically by DMSO and glucose

The infectivity of herpes simplex virus dna in rat embryo cells is enhanced synergistically by DMSO and glucose

Transfection of HSV DNA into rat embryo cells (previously an inefficient procedure) is enhanced more than 30-fold by simultaneous treatment with 20% glucose and 10% DMSO 4 h after transfection. The action of these compounds was efficient only when used synergistically.

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Bibliographische Detailangaben
Veröffentlicht in:Journal of virological methods 1983-01, Vol.6 (4), p.183-191
Hauptverfasser: Cameron, Iain R., Wilkie, Neil M., Macnab, Joan C.M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cells, Cultured
Cricetinae
Dimethyl Sulfoxide - pharmacology
DNA, Viral - genetics
DNA-plaquing
Embryo, Mammalian
gene transfer
Glucose - pharmacology
herpes simplex virus
HSV transformation
Rats
Simplexvirus - genetics
Transfection - drug effects
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Beschreibung
Zusammenfassung:Transfection of HSV DNA into rat embryo cells (previously an inefficient procedure) is enhanced more than 30-fold by simultaneous treatment with 20% glucose and 10% DMSO 4 h after transfection. The action of these compounds was efficient only when used synergistically.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(83)90045-9