Poliovirus and polio antibody assay in HEp-2 and Vero cell cultures

HEp-2 cell cultures were about three to 30 times more sensitive for poliovirus titration than Vero cells. Attenuated strains induced a complete cytopathic effect in HEp-2 but not in Vero cells. For polio antibody titration, HEp-2 and Vero cells were equally suitable. A high degree of sensitivity and...

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Veröffentlicht in:	Journal of biological standardization 1983-01, Vol.11 (2), p.91,IN1,95-94,IN1,97
Hauptverfasser:	Albrecht, P., Enterline, Joan C., Boone, Elizabeth J., Klutch, M.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Cells, Cultured Neutralization Tests poliovirus Poliovirus - immunology Poliovirus - isolation & purification Temperature Time Factors
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Beschreibung
Zusammenfassung:	HEp-2 cell cultures were about three to 30 times more sensitive for poliovirus titration than Vero cells. Attenuated strains induced a complete cytopathic effect in HEp-2 but not in Vero cells. For polio antibody titration, HEp-2 and Vero cells were equally suitable. A high degree of sensitivity and reproducibility of virus neutralization was achieved in tests utilizing a low virus dose and serum-virus incubation overnight at 36°C. Staining of infected trays with crystal violet obviated reading of viral CPE under the microscope and expedited the evaluation of larger-scale tests.
ISSN:	0092-1157
DOI:	10.1016/S0092-1157(83)80031-6