Action of testis graft from puromycin- or cAMP-pretreated donor embryos on the regression of Müllerian ducts in the female chick embryo

Female chick embryos grafted with a piece of embryonic testis manifest in a high percentage of cases a regression of their Müllerian ducts (MD) under the influence of a anti-Müllerian hormone (AMH) secreted by the graft. Puromycine or cAMP administered to grafted females reduced significantly the percentage of those presenting a MD regression. In the present work puromycin or cAMP was administered to the male graft-donor embryos and not to the grafted females as was done previously. A testis graft from a puromycin-pretreated donor was significantly less active on the MD than that from a normal embryo. It could be then concluded that puromycin acted at the level of the testis by inhibiting the AMH production. On the other hand, the treatment of donors with cAMP did not modify their testes with regard to their action on the grafted female MD. This showed that cAMP directly protected the MD against the AMH.