Unusual Monoclonal DNA Binding Immunoglobulin

SAWADA, S., IIJIMA, S., KUWANA, K., NISHINARITA, S., TAKEUCHI, J., SHIDA, M., KARASAKI, M. and AMAKI, I. Unusual Monoclonal DNA Binding Immunoglobulin. Tohoku J. exp. Med., 1983, 139 (3), 231-238 The monoclonal antibodies directed against DNA were produced by somatic cell hybridization with parental cells (SP-2) and spleen cells from nonimmunized autoimmune MRL/lpr mice. The immunoglobulins were recovered from the culture supernatant from hybridoma by a solid immunoadsorbent and antibody immunoprecipitation. The results from the specificities of DNA binding monoclonal immunoglobulins suggest that the antibodies to DNA have the antibody combining sites for both epitope of double stranded helicle and base of DNA and support the concept of the multiple antigen binding potentials of the hybridoma autoantibodies.