Augmentation of interleukin 2-activated cytotoxicity after treatment of cells with inhibitors of interferon production
Low-density human lymphocytes cultured with recombinant interleukin 2 (rIL-2) generated a high level of interferon(s) (IFN). Consistently more IFN including IFN-τ was produced during the first 3 days of culture with rIL-2 than during the subsequent 4 days. That ability was mainly associated with mep...
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Veröffentlicht in: | Cellular immunology 1991-04, Vol.133 (2), p.285-294 |
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Sprache: | eng |
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Zusammenfassung: | Low-density human lymphocytes cultured with recombinant interleukin 2 (rIL-2) generated a high level of interferon(s) (IFN). Consistently more IFN including IFN-τ was produced during the first 3 days of culture with rIL-2 than during the subsequent 4 days. That ability was mainly associated with mepacrine
+ cells and was decreased by low concentrations of leucine methyl ester (Leu-
O-Me) or ammonium chloride. Leu-
O-Me was employed either for the pretreatment of cells before the culture or as the additive to culture medium. The decrease in IFN production after pretreatment was associated with enhanced rIL-2-activated cytotoxicity. Similarly, 1 m
M of ammonium chloride or 1 m
M of Leu-
O-Me added to rIL-2 supplemented cultures for 3 days showed an association between inhibition of IFN-τ generation and increased activation of cytotoxic activity. Thus NK cells appear to regulate their own response to rIL-2 activation and the control mechanism seems to be associated with the ability of the cells to produce IFN(s) and possibly other cytokines. |
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ISSN: | 0008-8749 1090-2163 |
DOI: | 10.1016/0008-8749(91)90104-J |