Thromboxane Mediates Diapedesis After Ischemia by Activation of Neutrophil Adhesion Receptors Interacting With Basally Expressed Intercellular Adhesion Molecule-1

Ischemic injury is characterized by neutrophil (PMN)-endothelial cell adhesion and diapedesis associated with thromboxane (TX) generation. Neutrophil-endothelial cell interaction is regulated in part by the leukocyte adhesion receptor CD 18 glycoprotein complex and the endothelial intercellular adhesion molecule-1 (ICAM-1). This study tests the role of TX in ischemia-induced diapedesis and evaluates whether the diapedesis is regulated by neutrophil or endothelial adhesion receptors. Plasma derived from rabbit hind limbs made ischemic for 3 hours (n=6) and reperfused for 10 minutes had increased levels of TXB2 3,450 pg/ml, which was higher than sham rabbit (n=6) values of 653 pg/ml (p