Molecular cloning of the rat metallothionein 1 (MT-1) mRNA sequence

Starting with liver mRNA from cadmium chloride-treated rats, we constructed a cDNA library. A mouse metallothionein 1 (MT-1) cDNA restriction fragment (Durnam et al. 1980) was used as a hybridization probe to isolate from this library a cDNA (p2A10) which is a nearly complete copy of the rat MT-1 mR...

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Veröffentlicht in:	DNA (New York, N.Y.) N.Y.), 1983, Vol.2 (1), p.15-22
Hauptverfasser:	Andersen, R D, Birren, B W, Ganz, T, Piletz, J E, Herschman, H R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cadmium - pharmacology Cadmium Chloride Cloning, Molecular DNA - genetics Genes Hydrocortisone - pharmacology Liver - metabolism Metalloproteins - genetics Metallothionein - genetics Rats RNA, Messenger - biosynthesis RNA, Messenger - genetics
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Beschreibung
Zusammenfassung:	Starting with liver mRNA from cadmium chloride-treated rats, we constructed a cDNA library. A mouse metallothionein 1 (MT-1) cDNA restriction fragment (Durnam et al. 1980) was used as a hybridization probe to isolate from this library a cDNA (p2A10) which is a nearly complete copy of the rat MT-1 mRNA sequence. Southern analysis of rat DNA, using p2A10 as a probe, together with the screening of a rat genomic library, reveal that the rat MT-1 gene belongs to a family of related sequences. The rat MT-1 gene is inducible both by glucocorticoids and by metals.
ISSN:	0198-0238
DOI:	10.1089/dna.1.1983.2.15