Correcting a potential defect in an enzymatic cycle for NADP

Correcting a potential defect in an enzymatic cycle for NADP

An enzymatic cycle for NADP which uses as one of its enzymes glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has occasionally caused trouble due to failure to completely heat-kill this enzyme before the indicator step. It was found that a very small increase in pH was the cause of t...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical biochemistry 1983, Vol.128 (1), p.186-190
Hauptverfasser: Hintz, Carol S., Chi, Maggie M.-Y., Oliver, Lowry
Format: Artikel
Sprache:eng
Schlagworte:
Fluorometry - methods
Glucosephosphate Dehydrogenase - antagonists & inhibitors
Glutamate Dehydrogenase
Hot Temperature
Hydrogen-Ion Concentration
Leuconostoc - enzymology
NADP - analysis
Polyethylene Glycols
Serum Albumin, Bovine
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:An enzymatic cycle for NADP which uses as one of its enzymes glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has occasionally caused trouble due to failure to completely heat-kill this enzyme before the indicator step. It was found that a very small increase in pH was the cause of this. It was also found that the other two proteins present in the reagent greatly increase heat inactivation of the enzyme. The inactivation problem is completely overcome by keeping the pH below 7.2.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(83)90361-5