An enzymatic method for [ 32P]phosphoenolpyruvate synthesis

A convenient method for the enzymatic synthesis of [ 32P]phosphoenolpyruvate from [γ- 32P]ATP using partially pufified phosphoenolpyruvate carboxykinase from Escherichia coli is described. The synthesis was shown to convert essentially all the [γ- 32P]ATP to [ 32P]phosphoenolpyruvate, which was subs...

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Veröffentlicht in:	Analytical biochemistry 1983, Vol.128 (1), p.245-249
Hauptverfasser:	Mattoo, Roshan L., Waygood, E.Bruce
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, DEAE-Cellulose - methods Escherichia coli - enzymology Phosphoenolpyruvate - biosynthesis Phosphoenolpyruvate - chemical synthesis Phosphoenolpyruvate - isolation & purification Phosphoenolpyruvate Carboxykinase (GTP) - isolation & purification
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Beschreibung
Zusammenfassung:	A convenient method for the enzymatic synthesis of [ 32P]phosphoenolpyruvate from [γ- 32P]ATP using partially pufified phosphoenolpyruvate carboxykinase from Escherichia coli is described. The synthesis was shown to convert essentially all the [γ- 32P]ATP to [ 32P]phosphoenolpyruvate, which was subsequently separated from residual [γ- 32P]ATP and [ 32 P]P i by chromatography on AG-1-X8-bicarbonate resin.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(83)90372-X