A rapid affinity chromatography procedure for the isolation of glucose-6-phosphate dehydrogenase from human erythrocytes

A rapid affinity chromatography procedure for the isolation of glucose-6-phosphate dehydrogenase from human erythrocytes

The isolation of glucose-6-phosphate dehydrogenase from outdated human erythrocytes (type AB+) utilizing NADP + affinity chromatography and gel-permeation chromatography (Bio-Gel P-150) yields a fully active, homogeneous preparation. The procedure is faster and gives a higher yield than previous rep...

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Veröffentlicht in:Analytical biochemistry 1983-02, Vol.128 (2), p.312-316
Hauptverfasser: Craney, C.L., Goffredo, M.E.
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography, Affinity
Chromatography, Gel
erythrocytes
Erythrocytes - enzymology
glucose-6-phosphate dehydrogenase
Glucosephosphate Dehydrogenase - isolation & purification
Humans
man
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Zusammenfassung:The isolation of glucose-6-phosphate dehydrogenase from outdated human erythrocytes (type AB+) utilizing NADP + affinity chromatography and gel-permeation chromatography (Bio-Gel P-150) yields a fully active, homogeneous preparation. The procedure is faster and gives a higher yield than previous reports.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(83)90380-9